高血压与 2 型糖尿病患者表皮小纤维的减少有关,而与硬神经炎症无关。

IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Zhenchao Wang, Hanae Kushibiki, Takefusa Tarusawa, Sho Osonoi, Saori Ogasawara, Chinatsu Miura, Takanori Sasaki, Masaki Ryuzaki, Soroku Yagihashi, Hiroki Mizukami
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引用次数: 0

摘要

糖尿病多发性神经病(DPN)是一种多因素疾病,不仅与高血糖有关,还与高血压等循环障碍有关。众所周知,免疫系统与高血压之间存在密切的相互作用。在人类 DPN 的病理过程中,炎症反应是否与高血压有关仍不清楚。对尸检患者进行了评估:其中包括 7 名非糖尿病患者(nDM)、11 名患有高血压的非糖尿病患者(nDMHT)、6 名糖尿病患者(DM)和 9 名患有高血压和糖尿病的患者(DMHT)。采用免疫荧光染色法检测表皮内神经纤维密度(IENFD)。对解剖的鞍神经(SN)进行形态计量。使用抗-CD68和抗-CD206抗体进行双重免疫染色,评估真皮和内皮巨噬细胞浸润情况。与 nDM 相比,DM 的 IENFD 明显降低(p -/CD68+促炎巨噬细胞在 SN 中的浸润在 DM 中明显增加,与 nDM 相比,p +/CD68+抗炎巨噬细胞在 DM 中减少(p - 和 CD206+ 巨噬细胞与 MNFD 呈负相关(r = 0.42,p + 巨噬细胞浸润在所有组中相似)。糖尿病并发高血压明显增加了总弥散屏障厚度(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hypertension is associated with the reduction in epidermal small fibres independently of sural nerve inflammation in type 2 diabetic subjects.

Diabetic polyneuropathy (DPN) is a multifactorial disease associated not only with hyperglycaemia but also with circulatory disturbances such as hypertension. A close interaction between the immune system and hypertension is known. It remains unclear whether the inflammatory response is associated with hypertension in the pathology of human DPN. Autopsied patients were evaluated: 7 non-diabetic patients (nDM), 11 non-diabetic patients with hypertension (nDMHT), 6 patients with diabetes (DM) and 9 patients with hypertension and diabetes (DMHT). Intraepidermal nerve fibre density (IENFD) was examined by immunofluorescent staining. Dissected sural nerve (SNs) were morphometrically quantified. Dermal and endoneurial macrophage infiltration was evaluated by double immunostaining using anti-CD68 and anti-CD206 antibodies. IENFD was significantly decreased in DM compared to nDM (p < 0.05) and was further decreased in DMHT (p < 0.05). Myelinated nerve fibre density (MNFD) in the SN was significantly decreased in DM compared with nDM (p < 0.05) and further decreased in DMHT (p < 0.01 vs. DM). The infiltration of CD206-/CD68+ proinflammatory macrophages in the SN was significantly increased in DM compared to nDM (p < 0.05), whilst the number of CD206+/CD68+ anti-inflammatory macrophages was decreased in DM (p < 0.05). Hypertension had no impact on macrophage infiltration. The ratio of CD206- and CD206+ macrophage was negatively correlated with MNFD (r = 0.42, p < 0.05) but not IENFD (r = 0.30, p = 0.09). Dermal CD206+ macrophage infiltration was similar amongst all groups. Diabetes complicated by hypertension significantly increased the total diffusion barrier thickness (p < 0.01 vs. DM). Total diffusion barrier thickness was inversely correlated with both IENFD (r = -0.59, p < 0.01) and MNFD (r =-0.62, p < 0.01). Our results suggest that vascular factors and inflammation might be synergistically involved in pathological changes in human diabetic patients through different mechanisms.

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来源期刊
Journal of Neurochemistry
Journal of Neurochemistry 医学-神经科学
CiteScore
9.30
自引率
2.10%
发文量
181
审稿时长
2.2 months
期刊介绍: Journal of Neurochemistry focuses on molecular, cellular and biochemical aspects of the nervous system, the pathogenesis of neurological disorders and the development of disease specific biomarkers. It is devoted to the prompt publication of original findings of the highest scientific priority and value that provide novel mechanistic insights, represent a clear advance over previous studies and have the potential to generate exciting future research.
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