qTaHa-5DL：调节小麦籽粒硬度的另一个主要 QTL。

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-10-28 DOI:10.1186/s12870-024-05731-z

Xiangyun Fan, Peng Jiang, Yi He, Xu Zhang, Guihong Yu, Meixue Zhou, Peng Zhang

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引用次数: 0

摘要

谷粒硬度对谷粒品质和小麦的最终用途有重要影响。本研究利用收集的 103 个普通小麦种质和 194 个品系的 DH 群体来鉴定谷粒硬度的新数量性状位点（QTL）。在不同地点和年份，在染色体 5D 上发现了两个稳定的遗传位点，其中一个是 5DS 上的 Ha 位点，小麦谷粒硬度的主要基因 Puroindolines 就位于该位点。另一个位点 qTaHa-5DL 也对谷粒硬度指数（HI）有显著影响，当 Puroindolines 为野生型时，HI 从约 20 增加到约 45，硬度类型从软变为混合型。针对 qTaHa-5DL 开发了一种竞争性等位基因特异性 PCR（KASP）标记，并在 184 个育种品系中证实了该 QTL 的有效性，标记 K-Ha5DL 将小麦分为两个不同的类别。这一新的 QTL 可有效用于选择软质或中硬质小麦。

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qTaHa-5DL: another major QTL regulating wheat grain hardness.

Grain hardness has important effects on grain quality and the end-use of wheat. In this study, a collection of 103 common wheat germplasms and a DH population of 194 lines were used to identify new quantitative trait loci (QTL) for grain hardness. Two stable genetic loci on chromosome 5D were detected under different locations and years with one of them being the Ha locus on 5DS where the major gene Puroindolines for wheat grain hardness is located. Another locus of qTaHa-5DL also showed a significant impact on grain hardness index (HI) with HI increasing from ~ 20 to ~ 45 and hardness type changing from soft to mixed when Puroindolines are wild type. A kompetitive allele-specific PCR (KASP) marker for qTaHa-5DL was developed and the effectiveness of the QTL was confirmed in 184 breeding lines with the marker K-Ha5DL dividing wheat into two distinct categories. This new QTL can be effectively used to select soft or medium hard wheat.

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.