表面等离子体共振成像在流感病毒高通量检测中的应用。

IF 2.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Annals of Clinical Biochemistry Pub Date : 2024-11-06 DOI:10.1177/00045632241297819

Haixiang Zhang, Jingying Sun, Chunyan Guo, Qing Feng, Yan Li, Xiangrong Zhao, Lijun Sun, Cuixiang Xu

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引用次数: 0

摘要

目的：评估 SPRi 单克隆抗体芯片在复杂混合物中检测流感病毒抗原的应用效果：评估 SPRi 单克隆抗体（mAb）芯片在复杂混合物中检测流感病毒抗原的应用效果：方法：制备了 115 株针对不同亚型（H1N1、H5N1、A1、A3、B、H7N9、H9N2 和 H3N2）流感病毒的 mAb。利用表面等离子体共振成像（SPRi）技术制备了抗流感病毒的 mAbs 芯片，用于检测流感病毒上清液，并与传统的抗原捕获 ELISA 方法进行了比较：比较研究表明，传统的抗原捕获 ELISA 方法灵敏度更高（86.8% (46/53) vs. 46.5% (46/99)；z = 4.84，P < 0.001），而 SPRi 芯片方法的特异性明显更高（56.3% (9/16) vs. 14.5% (9/62)；z = 3.54，P < 0.001）。SPRi芯片检测流感病毒抗体的方法能很好地反映流感病毒抗原和抗体的特异性结合特征：结论：SPRi mAb 芯片可用于检测流感病毒上清液等复杂混合物中的特定病原微生物或病毒蛋白。结论：SPRi mAb 芯片可用于流感病毒上清液等复杂混合物中特定病原微生物或病毒蛋白的检测，具有无标记、实时、高通量、特异性强等显著优势，可在疾病诊断和传染病防控中发挥重要作用。

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Application of surface Plasmon resonance imaging in the high-throughput detection of influenza virus.

Objective: To evaluate the application effect of SPRi monoclonal antibody (mAb) chip in the detection of influenza virus antigen in complex mixtures.

Methods: A total of 115 strains of mAbs against different subtypes (H1N1, H5N1, A1, A3, B, H7N9, H9N2, and H3N2) of influenza virus were prepared. The chip of mAbs against influenza virus was prepared by surface plasmonic resonance imaging (SPRi) technology, which was used for the detection of influenza virus supernatant, and compared with the traditional antigen capture ELISA method.

Results: Comparative studies have shown that traditional antigen capture ELISA methods have a higher sensitivity (86.8% (46/53) vs. 46.5% (46/99); z = 4.84, P < .001), while the SPRi chip methods present a significantly higher specificity (56.3% (9/16) vs. 14.5% (9/62); z = 3.54, P < .001). The SPRi chip detection method for influenza virus antibodies can well reflect the specific binding characteristics of influenza virus antigens and antibodies.

Conclusion: The SPRi mAb chip can be used for the detection of specific pathogenic microorganisms or viral proteins in complex mixtures such as influenza virus supernatant. It has significant advantages of label free, real-time, high-throughput, and good specificity, and can play an important role in disease diagnosis and infectious disease prevention and control.

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).