对旅行相关分离物的监测揭示了非流行性霍乱弧菌的多样性。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
Lia Bote, Alyce Taylor-Brown, Mailis Maes, Danielle J Ingle, Mary Valcanis, Benjamin P Howden, Nicholas R Thomson
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引用次数: 0

摘要

霍乱弧菌是一种存在于水生环境中的革兰氏阴性细菌,是霍乱的病原体,其特征是急性水样腹泻和严重脱水。霍乱给全球健康造成了巨大负担,估计每年有 130-500 万病例,目前的大流行是由 O1 El Tor 生物型的毒素系引起的,称为第七次大流行 El Tor(7PET),目前仍在持续。尽管人们知道非 7PET 世系可引起零星疾病,但对这些非流行 世系的传播却知之甚少。2005 年至 2017 年间,从从印度尼西亚返回澳大利亚的旅行者身上获得了 34 个霍乱弧菌分离株。对这些分离物进行了全基因组测序,将其与 883 个分离物放入全球系统发育背景中,并筛选了与抗菌药耐药性和毒力相关的已知基因。分析结果显示,30 个分离株属于非 7PET 系,4 个属于 7PET 系。7PET 和非 7PET 分离物均携带对四环素类和氟喹诺酮类等霍乱治疗常用抗生素的耐药基因。非 7PET 分离物中也存在多种毒力因子,其中两个分离物明显携带毒素调控的柔毛基因,这主要是 7PET 霍乱弧菌在肠道定殖的原因。这项研究表明了旅行在霍乱弧菌流行系和非流行系远距离携带中的作用,以及哨点旅行监测如何丰富我们对霍乱弧菌多样性的了解,揭示具有不同疾病潜能的不同系传播的新生物学特性,并阐明在监测数据有限的流行地区是否存在疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Surveillance of travel-associated isolates elucidates the diversity of non-pandemic Vibrio cholerae.

Vibrio cholerae is a Gram-negative bacterium found in aquatic environments and is the aetiological agent of cholera, characterized by acute watery diarrhoea and severe dehydration. Cholera presents a significant global health burden of an estimated 1.3-5 million annual cases, with the current pandemic caused by a toxigenic lineage of the O1 El Tor biotype called seventh pandemic El Tor (7PET) that is still ongoing. Whilst it is known that non-7PET lineages can cause sporadic disease, little is known about the transmission of these non-epidemic lineages. Thirty-four V. cholerae isolates were obtained from travellers returning from Indonesia to Australia between 2005 and 2017. These were whole genome sequenced, placed into a global phylogenetic context with 883 isolates, and screened for known genes associated with antimicrobial resistance and virulence. This analysis revealed that 30 isolates fell within non-7PET lineages and four within the 7PET lineage. Both 7PET and non-7PET isolates carried genes for resistance to antibiotics that are commonly used in cholera treatment such as tetracyclines and fluoroquinolones. Diverse virulence factors were also present in non-7PET isolates, with two isolates notably carrying toxin-coregulated pilus genes, which are primarily responsible for intestinal colonization in 7PET V. cholerae. This study demonstrates the role of travel in long-range carriage of epidemic and non-epidemic lineages of V. cholerae, and how sentinel travel surveillance can enrich our knowledge of V. cholerae diversity, reveal new biology about the spread of diverse lineages with differing disease potential and illuminate disease presence in endemic regions with limited surveillance data.

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来源期刊
Microbial Genomics
Microbial Genomics Medicine-Epidemiology
CiteScore
6.60
自引率
2.60%
发文量
153
审稿时长
12 weeks
期刊介绍: Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.
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