绘制甘蓝型油菜对水分胁迫耐受性的数量性状位点(QTL)图。

IF 2.3 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Genome Pub Date : 2024-12-01 Epub Date: 2024-10-17 DOI:10.1139/gen-2023-0127
Samadhi B Jayarathna, Harmeet S Chawla, Mohammed M Mira, Robert W Duncan, Claudio Stasolla
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引用次数: 0

摘要

甘蓝型油菜(Brassica napus L.)植物从种子萌发到结籽的整个生命周期对水分胁迫条件都很敏感。本研究旨在鉴定与油菜耐受 10%聚乙二醇-6000(PEG-6000)模拟水胁迫有关的数量性状基因座(QTL)。这项研究使用了两个加倍单倍体群体,每个群体由 150 个基因型组成。在没有 PEG-6000 的环境条件下(对照)或有 PEG-6000 的环境条件下(胁迫),将处于两片真叶发育阶段的植株生长 48 小时,并计算每个基因型的干旱胁迫指数。使用 Brassica Infinium 90K SNP BeadChip 阵列对所有基因型及其亲本进行基因分型。利用包容性复合间隔图谱鉴定 QTL。在 6 条染色体(A2、A3、A4、A9、C3 和 C7)上确定了 6 个与水胁迫耐受性相关的 QTL 和 12 个推定 QTL。在所有已鉴定的 QTL 中,共鉴定出 2154 个耐水胁迫候选基因。其中,根据九个功能注释,确定了 213 个与水胁迫(由 PEG-6000 施加)耐受性直接相关的基因。这些结果可纳入未来的育种计划中,以选育出有能力有效应对水胁迫的植物材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mapping of quantitative trait loci (QTL) in Brassica napus L. for tolerance to water stress.

Brassica napus L. plants are sensitive to water stress conditions throughout their life cycle from seed germination to seed setting. This study aims at identifying quantitative trait loci (QTL) linked to B. napus tolerance to water stress mimicked by applications of 10% polyethylene glycol-6000 (PEG-6000). Two doubled haploid populations, each consisting of 150 genotypes, were used for this research. Plants at the two true leaf stage of development were grown in the absence (control) or presence (stress) of PEG-6000 under controlled environmental conditions for 48 h, and the drought stress index was calculated for each genotype. All genotypes, along with their parents, were genotyped using the Brassica Infinium 90K SNP BeadChip Array. Inclusive composite interval mapping was used to identify QTL. Six QTL and 12 putative QTL associated with water stress tolerance were identified across six chromosomes (A2, A3, A4, A9, C3, and C7). Collectively, 2154 candidate genes for water stress tolerance were identified for all the identified QTL. Among them, 213 genes were identified as being directly associated with water stress (imposed by PEG-6000) tolerance based on nine functional annotations. These results can be incorporated into future breeding initiatives to select plant material with the ability to cope effectively with water stress.

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来源期刊
Genome
Genome 生物-生物工程与应用微生物
CiteScore
5.30
自引率
3.20%
发文量
42
审稿时长
6-12 weeks
期刊介绍: Genome is a monthly journal, established in 1959, that publishes original research articles, reviews, mini-reviews, current opinions, and commentaries. Areas of interest include general genetics and genomics, cytogenetics, molecular and evolutionary genetics, developmental genetics, population genetics, phylogenomics, molecular identification, as well as emerging areas such as ecological, comparative, and functional genomics.
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