Mia L Abramsson, Louise J Persson, Frank Sobott, Erik G Marklund, Michael Landreh
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引用次数: 0
摘要
原生质谱法(nMS)通过 "软 "电喷雾离子化(ESI)保留非共价相互作用,使人们能够深入了解生物大分子在原生状态下的结构和动力学。对于原生蛋白质,获得的电荷数量与表面积和质量成比例。在这里,我们探讨了带高负电荷的 DNA 对蛋白质复合物的 ESI 电荷的影响,结果发现质量电荷比降低了,而且变化更大。纯 DNA 集合体的电荷状态分布显示出比蛋白质更低的质量电荷比,这是因为它们在气相中的密度更大,而蛋白质-DNA 复合物的电荷还会受到 ESI 电荷分布、离子配对事件和 DNA 成分塌缩的影响。我们的研究结果表明,蛋白质-DNA 复合物的结构特征会导致电荷状态低于蛋白质的预期电荷状态。
Charging of DNA Complexes in Positive-Mode Native Electrospray Ionization Mass Spectrometry.
Native mass spectrometry (nMS) provides insights into the structures and dynamics of biomacromolecules in their native-like states by preserving noncovalent interactions through "soft" electrospray ionization (ESI). For native proteins, the number of charges that are acquired scales with the surface area and mass. Here, we explore the effect of highly negatively charged DNA on the ESI charge of protein complexes and find a reduction of the mass-to-charge ratio as well as a greater variation. The charge state distributions of pure DNA assemblies show a lower mass-to-charge ratio than proteins due to their greater density in the gas phase, whereas the charge of protein-DNA complexes can additionally be influenced by the distribution of the ESI charges, ion pairing events, and collapse of the DNA components. Our findings suggest that structural features of protein-DNA complexes can result in lower charge states than expected for proteins.
期刊介绍:
The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role.
Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives