微RNA富集于COVID-19基因组风险区,其血液水平与感染SARS-CoV-2的癌症患者的COVID-19预后相关

IF 27.7 1区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Simone Anfossi, Faezeh Darbaniyan, Joseph Quinlan, Steliana Calin, Masayoshi Shimizu, Meng Chen, Paola Rausseo, Michael Winters, Elena Bogatenkova, Kim-Anh Do, Ivan Martinez, Ziyi Li, Loredana Antal, Tudor Rares Olariu, Ignacio Wistuba, George A. Calin
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引用次数: 0

摘要

癌症患者更容易受到COVID-19侵袭性病程的影响。开发生物标记物来识别COVID-19相关死亡高风险癌症患者,有助于确定哪些患者需要早期临床干预。寄存在与COVID-19侵袭性风险相关的基因组区域中的miRNA可能是临床结果的潜在生物标志物。德克萨斯大学 MD 安德森癌症中心收集了受 COVID-19 影响的癌症患者(128 人)的血浆样本。血清样本来自罗马尼亚蒂米什瓦拉市临床急救教学医院的接种过疫苗的健康人(23 人)。研究人员采用硅定位克隆方法来鉴定 COVID-19 风险相关基因组区域是否存在 miRNAs:CORSAIRs(COVID-19 RiSk AssocIated genomic Regions)。通过 RT-qPCR 测定了 miRNA 水平。我们发现,miRNA 在 CORSAIR 中富集。血浆中低水平的hsa-miR-150-5p和hsa-miR-93-5p与较高的COVID-19相关死亡有关。hsa-miR-92b-3p 的水平与 SARS-CoV-2 检测阳性有关。体外 TLR7/8 和 T 细胞受体(TCR)介导的活化后,外周血单核细胞(PBMC)分泌的 hsa-miR-150-5p、hsa-miR-93-5p 和 hsa-miR-92b-3p 增加。在接种辉瑞-生物技术公司生产的 COVID-19 疫苗第二剂后的 1 至 9 个月期间,健康人血清样本中这三种 miRNA 水平的升高得到了测定。人类气道上皮细胞感染 SARS-CoV-2 后,其分泌的细胞外囊泡内的 miRNA 水平会受到影响。MiRNA 在 CORSAIR 中富集。血浆中的 miRNA 水平可作为潜在的血液生物标志物,用于预测癌症患者与 COVID-19 相关的死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MicroRNAs are enriched at COVID-19 genomic risk regions, and their blood levels correlate with the COVID-19 prognosis of cancer patients infected by SARS-CoV-2
Cancer patients are more susceptible to an aggressive course of COVID-19. Developing biomarkers identifying cancer patients at high risk of COVID-19-related death could help determine who needs early clinical intervention. The miRNAs hosted in the genomic regions associated with the risk of aggressive COVID-19 could represent potential biomarkers for clinical outcomes. Plasma samples were collected at The University of Texas MD Anderson Cancer Center from cancer patients (N = 128) affected by COVID-19. Serum samples were collected from vaccinated healthy individuals (n = 23) at the Municipal Clinical Emergency Teaching Hospital in Timisoara, Romania. An in silico positional cloning approach was used to identify the presence of miRNAs at COVID-19 risk-associated genomic regions: CORSAIRs (COvid-19 RiSk AssocIated genomic Regions). The miRNA levels were measured by RT-qPCR. We found that miRNAs were enriched in CORSAIR. Low plasma levels of hsa-miR-150-5p and hsa-miR-93-5p were associated with higher COVID-19-related death. The levels of hsa-miR-92b-3p were associated with SARS-CoV-2 test positivity. Peripheral blood mononuclear cells (PBMC) increased secretion of hsa-miR-150-5p, hsa-miR-93-5p, and hsa-miR-92b-3p after in vitro TLR7/8- and T cell receptor (TCR)-mediated activation. Increased levels of these three miRNAs were measured in the serum samples of healthy individuals between one and nine months after the second dose of the Pfizer-BioNTech COVID-19 vaccine. SARS-CoV-2 infection of human airway epithelial cells influenced the miRNA levels inside their secreted extracellular vesicles. MiRNAs are enriched at CORSAIR. Plasma miRNA levels can represent a potential blood biomarker for predicting COVID-19-related death in cancer patients.
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来源期刊
Molecular Cancer
Molecular Cancer 医学-生化与分子生物学
CiteScore
54.90
自引率
2.70%
发文量
224
审稿时长
2 months
期刊介绍: Molecular Cancer is a platform that encourages the exchange of ideas and discoveries in the field of cancer research, particularly focusing on the molecular aspects. Our goal is to facilitate discussions and provide insights into various areas of cancer and related biomedical science. We welcome articles from basic, translational, and clinical research that contribute to the advancement of understanding, prevention, diagnosis, and treatment of cancer. The scope of topics covered in Molecular Cancer is diverse and inclusive. These include, but are not limited to, cell and tumor biology, angiogenesis, utilizing animal models, understanding metastasis, exploring cancer antigens and the immune response, investigating cellular signaling and molecular biology, examining epidemiology, genetic and molecular profiling of cancer, identifying molecular targets, studying cancer stem cells, exploring DNA damage and repair mechanisms, analyzing cell cycle regulation, investigating apoptosis, exploring molecular virology, and evaluating vaccine and antibody-based cancer therapies. Molecular Cancer serves as an important platform for sharing exciting discoveries in cancer-related research. It offers an unparalleled opportunity to communicate information to both specialists and the general public. The online presence of Molecular Cancer enables immediate publication of accepted articles and facilitates the presentation of large datasets and supplementary information. This ensures that new research is efficiently and rapidly disseminated to the scientific community.
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