乙醇对 Lumbriculus variegatus 的行为影响与浓度和时间有关

IF 2.4 4区 心理学 Q2 BEHAVIORAL SCIENCES
Aidan Seeley, Romessa Mahmood, Caitlin Bellamy, Elis G. Roome, Benjamin S. Williams, Nia A. Davies, Melisa J. Wallace
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引用次数: 0

摘要

乙醇是世界上使用最广泛的药物之一。乙醇能诱导无脊椎动物模式生物(如秀丽隐杆线虫和黑腹果蝇)产生深刻的生理和行为反应。Lumbriculus variegatus(无脊椎动物,无毛类)是一种水生蠕虫,对常见药物有行为反应,因此有可能用于药理学研究。乙醇对这种生物的影响尚不清楚。在本研究中,我们研究了急性暴露于乙醇(0-500 mM)对变节鞘氨醇的刻板运动和运动活动的影响,并研究了乙醇对变节鞘氨醇的浓度(0-500 mM)和时间依赖性(0-210 分钟)效应。我们发现,≥250 mM的乙醇可逆地降低触觉刺激诱发定型运动的能力,即身体反转和螺旋游动以及运动活动(p < 0.05,N = 8)。我们还发现,暴露于≥250 mM乙醇中2分钟可迅速诱导稳态运动减弱(p < 0.05, N = 11),并证实乙醇可被L. variegatus组织吸收。此外,我们还观察到暴露于 500 mM 乙醇 150 分钟后的急性乙醇耐受性(p < 0.05,N = 24)。本研究首次报道了乙醇对变节龙的行为影响。我们的研究结果表明,这是一种可用于乙醇研究的模式生物,为其在药理学研究中的应用提供了进一步的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Concentration- and time-dependent behavioural effects of ethanol on Lumbriculus variegatus

Ethanol is one of the most widely used drugs in the world. Ethanol induces profound physiological and behavioural responses in invertebrate model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus (Annelida, Oligochaete) is an aquatic worm which shows behavioural responses to common drugs and thus is potentially useful in pharmacological research. The effects of ethanol are unknown in this organism. In this study, we examine the effects of acute exposure to ethanol (0–500 mM) on the stereotypical movements and locomotor activity of L. variegatus and examine the concentration- (0–500 mM) and time-dependent (0–210 min) effects of ethanol in L. variegatus. We show that ≥250 mM ethanol reversibly reduced the ability of tactile stimulation to elicit stereotypical movements, namely body reversal and helical swimming and locomotor activity (p < 0.05, N = 8). We also found that 2 min of exposure to ≥250 mM ethanol rapidly induces steady-state hypokinesis (p < 0.05, N = 11) and confirm ethanol absorption into L. variegatus tissues. Additionally, we also observed acute ethanol tolerance after 150 min of exposure to 500 mM ethanol (p < 0.05, N = 24). This study is the first to report the behavioural effects of ethanol in L. variegatus. Our results show that this is a model organism for use in ethanol studies, providing further evidence for its utility in pharmacological research.

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来源期刊
Genes Brain and Behavior
Genes Brain and Behavior 医学-行为科学
CiteScore
6.80
自引率
4.00%
发文量
62
审稿时长
4-8 weeks
期刊介绍: Genes, Brain and Behavior was launched in 2002 with the aim of publishing top quality research in behavioral and neural genetics in their broadest sense. The emphasis is on the analysis of the behavioral and neural phenotypes under consideration, the unifying theme being the genetic approach as a tool to increase our understanding of these phenotypes. Genes Brain and Behavior is pleased to offer the following features: 8 issues per year online submissions with first editorial decisions within 3-4 weeks and fast publication at Wiley-Blackwells High visibility through its coverage by PubMed/Medline, Current Contents and other major abstracting and indexing services Inclusion in the Wiley-Blackwell consortial license, extending readership to thousands of international libraries and institutions A large and varied editorial board comprising of international specialists.
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