芪蛭降糖胶囊对心肌梗死后小鼠血清非靶向代谢组学分析

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2024-10-10 DOI:10.1016/j.jpba.2024.116516

Yingfei Wang , Shijiao Zhang , Yingying Ge , Chunxia Miao , Benrong Liu , Tao Yang , Xiangjun Qiu , Wenchao Ou

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引用次数: 0

摘要

芪蛭降糖胶囊由黄芪、人参、附子、丹参、附子、泽泻、玉竹 11 味药组成、桂枝、红花、香附、陈皮，是一种标准化的商业配方，旨在解决阳虚问题，恢复心气平衡。此外，QLQX 还具有活血通络、生津止渴的功效，可用于冠心病、高血压引起的轻中度充血性心力衰竭等心血管疾病。进一步研究 QLQX 对心肌梗塞后小鼠心功能的影响。对心肌梗塞模型小鼠灌胃适当剂量的 QLQX，动物实验结束后通过 UHPLC-Q-Exactive LC-MS 对样品进行分析。采用液质联用仪收集样本，然后利用代谢组学分析进一步分析相应的代谢物和代谢途径。结果发现了9种不同的代谢物，其中15种在使用QLQX干预后上调，4种下调。然后通过 KEGG 富集途径气泡图分析代谢途径，得到了 4 条代谢途径，结合筛选出的代谢物一起分析，最终发现了 2,5-二羟基苯磺酸和邻甲酚硫酸盐这两种差异代谢物。甘油磷脂代谢途径与其余 7 个差异代谢物密切相关，该途径可能是 QLQX 影响小鼠心脏功能的重要途径。

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Serum untargeted metabolomics analysis of mice after myocardial infarction affected by qiliqiangxin capsule

Qiliqiangxin (QLQX) capsule consists of 11 herbs, namely Huang qi (astragalus membranaceus), Ren shen (ginseng), Fu zi (radix aconiti carmichaeli), Dan shen (salvia miltiorrhiza), Ting li zi (lepidium seed), Ze xie (rhizoma alismatis), Yu zhu (radix polygonati officinalis), Gui zhi (cassia twig), Hong hua (carthamus tinctorious), Xiang jia Pi (cortex periplocae), Chen Pi (pericarpium citri reticulatae), and it is a standardized commercial formula designed to address yang deficiency and to restore the balance of qi in the heart. QLQX is also known to invigorate the blood and promote the circulation of the blood and to promote the use of fluids to relieve water retention and edema, and can be used in cardiovascular diseases such as mild to moderate congestive heart failure resulting from coronary artery disease and hypertension. The further research on the effect of QLQX on cardiac function in mice after myocardial infarction was manipulated. QLQX was given to mice in myocardial infarction model by gavage with appropriate dosage and the samples were analyzed at the end of the animal experiments through the UHPLC-Q-Exactive LC-MS. The liquid mass spectrometry was used to collect and followed by further analysis of the corresponding metabolites and metabolic pathways using metabolomics analysis. As a result, 9 differential metabolites were identified, with 15 being up-regulated and 4 down-regulated following intervention with QLQX. Then the metabolic pathways by KEGG enrichment pathway bubble diagram was analyzed, and 4 metabolic pathways were obtained, and combined with the metabolites that had been screened and analyzed together, finally the two differential metabolites, 2,5-Dihydroxybenzenesulfonic Acid and o-Cresol sulfate were found. The Glycerophospholipid metabolism pathway was closely related to the remaining seven differential metabolites, and the pathway might be an important pathway related to the effects of QLQX on cardiac function in mice.

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.