采用两步底物添加策略，在大肠杆菌中利用大孔吸附树脂从白藜芦醇中生物合成紫檀芪

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI:10.1002/bit.28865

Bao Yingling, Wu Xueqin, Chen Xiaolong

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引用次数: 0

摘要

紫檀芪（PST）是白藜芦醇（RSV）的一种 3',5'-O-甲基化衍生物，是一种强效的天然抗氧化剂，由一些植物作为防御化合物微量生成。它具有多种促进健康的活性，如抗癌、抗病毒和抗菌作用。由于从植物中提取 PST 所面临的挑战，因此大规模生物合成 PST 至关重要。本研究旨在开发一种利用工程大肠杆菌菌株以 RSV 为前体生产 PST 的高效方法。我们引入了一种两步底物添加策略，结合大孔吸附树脂（MAR）上的固定化 RSV（IMRSV）来提高 PST 的产量。我们选择了五种固定 RSV 的 MAR，并优化了合成 PST 的底物添加策略和发酵参数。与一步添加游离 RSV 的对照组相比，PST 的最高浓度达到 403 ± 9 mg/L，提高了 239%。在 3 升生物反应器中，PST 滴度达到 395 ± 24 mg/L。总之，两步底物添加系统与 IMRSV 的结合是一种经济、工业化生产 PST 的可行方法。

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Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy

Pterostilbene (PST), a 3’,5’-O-methylated derivative of resveratrol (RSV), is a potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health-promoting activities, such as anticancer, antiviral, and antimicrobial effects. Large-scale biosynthesis of PST is crucial due to the challenges associated with extracting it from plants. This study aims to develop an efficient method for PST production using an engineered Escherichia coli strain by feeding RSV as a precursor. We introduced a two-step substrate addition strategy combined with immobilized RSV (IMRSV) on macroporous adsorption resin (MAR) to enhance PST production. Five MARs were selected for RSV immobilization, and the substrate addition strategy and fermentation parameters for PST synthesis were optimized. A maximum PST concentration of 403 ± 9 mg/L was achieved, representing a 239% increase over the control, which in a one-step addition of free RSV. The PST titer reached 395 ± 24 mg/L in a 3-L bioreactor. In conclusion, the combination of a two-step substrate addition system and IMRSV is a promising approach for the economical and industrial-scale production of PST.

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

期刊介绍： Biotechnology & Bioengineering publishes Perspectives, Articles, Reviews, Mini-Reviews, and Communications to the Editor that embrace all aspects of biotechnology. These include: -Enzyme systems and their applications, including enzyme reactors, purification, and applied aspects of protein engineering -Animal-cell biotechnology, including media development -Applied aspects of cellular physiology, metabolism, and energetics -Biocatalysis and applied enzymology, including enzyme reactors, protein engineering, and nanobiotechnology -Biothermodynamics -Biofuels, including biomass and renewable resource engineering -Biomaterials, including delivery systems and materials for tissue engineering -Bioprocess engineering, including kinetics and modeling of biological systems, transport phenomena in bioreactors, bioreactor design, monitoring, and control -Biosensors and instrumentation -Computational and systems biology, including bioinformatics and genomic/proteomic studies -Environmental biotechnology, including biofilms, algal systems, and bioremediation -Metabolic and cellular engineering -Plant-cell biotechnology -Spectroscopic and other analytical techniques for biotechnological applications -Synthetic biology -Tissue engineering, stem-cell bioengineering, regenerative medicine, gene therapy and delivery systems The editors will consider papers for publication based on novelty, their immediate or future impact on biotechnological processes, and their contribution to the advancement of biochemical engineering science. Submission of papers dealing with routine aspects of bioprocessing, description of established equipment, and routine applications of established methodologies (e.g., control strategies, modeling, experimental methods) is discouraged. Theoretical papers will be judged based on the novelty of the approach and their potential impact, or on their novel capability to predict and elucidate experimental observations.