通过信号探针杂交以 PCR 和免清洗方式检测血清 miRNA

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2024-10-13 DOI:10.1002/bit.28859

Haruka Uno, Hiyori Takeuchi, Ishin Abe, Tomoko Yoshino, Tomoyuki Taguchi, Yuko Hirakawa, Tadashi Matsunaga, Tsuyoshi Tanaka

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引用次数: 0

摘要

检测血清中的微RNA（miRNA）是一种有效的癌症诊断液体活检技术。然而，传统的诊断方法耗时且复杂。因此，在这项研究中，我们建立了一种基于信号探针的 DNA 微阵列系统来检测 miRNA。信号探针无需进行 PCR、荧光标记和洗涤。我们利用不同的 miRNA 设计了四种探针，作为诊断癌症的标志物。所开发的系统适用于各种 miRNA，无论其目标长度（18-26-mer）和 GC 含量（36%-89%）如何。所有检测均在 40 分钟内完成。总之，我们基于信号探针的 DNA 杂交系统有助于简单快速地检测血清 miRNA，而无需基因扩增、荧光标记和洗涤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

PCR- and wash-free detection of serum miRNA via signaling probe hybridization

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PCR- and wash-free detection of serum miRNA via signaling probe hybridization

Detection of microRNAs (miRNAs) in the serum is an effective liquid biopsy technique for cancer diagnosis. However, conventional diagnostic methods are time-consuming and complex. Therefore, in this study, we established a signaling probe-based DNA microarray system for miRNA detection. PCR, fluorescence labeling, and washing are not necessary for signaling probes. Four probes were designed using different miRNAs as diagnostic cancer markers. The developed system is useful for various miRNAs, regardless of their target lengths (18–26-mer) and GC content (36%–89%). Here, all the assays were performed within 40 min. Overall, our signaling probe-based DNA hybridization system facilitates the simple and rapid detection of serum miRNAs without the need for gene amplification, fluorescence labeling and washing.

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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