碱性溶液、碳水化合物酶和生物质：溶剂对棕榈蛋白提取物的提取效率以及蛋白质和氨基酸谱的影响

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2024-09-26 DOI:10.1016/j.fbp.2024.09.017

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引用次数: 0

摘要

研究了使用不同的碱性溶液、碳水化合物酶以及改变生物质与溶剂的体积比（生物质：溶剂）对棕榈叶（红藻）蛋白质富集提取物中蛋白质含量、蛋白质和生物质产量回收率的影响。评估了使用碱性溶液，即 NaOH、KOH、Ca(OH)2 和 Na2CO3（0.12 M）以及（或不使用）碳水化合物酶 B、BD 和 L（分别含有木聚糖酶、木聚糖酶和纤维素酶的混合物以及纤维素酶、木聚糖酶和聚半乳糖醛酸酶活性的组合）提取蛋白质的情况。采用主成分分析法评估了不同碱性溶剂、碳水化合物酶和生物质：溶剂变化对提取效率的相关性（如有）。使用 KOH 辅助萃取和碳水化合物酶 BD，蛋白质含量最高（56.64 ± 0.43 %（w/w））。在使用 NaOH 和碳水化合物酶 BD 辅助提取时，蛋白质和生物量的回收率最高（分别为 70.65 ± 1.38 % 和 21.99 ± 0.43 %（重量比））。电泳分析、氨基酸谱分析和氨基酸评分分析因提取蛋白质所用的碱性溶剂不同而存在差异。在蛋白质提取过程中适当选择碱性溶剂、碳水化合物酶和生物量：体积组合，有助于从棕榈属植物中生产出成分更优的优质浓缩蛋白质。

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Impact of alkaline solution, carbohydrase and biomass:solvent on extraction efficiency, and protein and amino acid profiles of Palmaria palmata protein extracts

The impact of using different alkaline solutions, carbohydrases and changing the biomass to solvent volume ratio (biomass:solvent) on protein content, protein and biomass yield recovery in protein enriched extracts from Palmaria palmata (red seaweed) was studied. Protein extraction with alkaline solutions, i.e., NaOH, KOH, Ca(OH)₂ and Na₂CO₃ (at 0.12 M) along with (and without) carbohydrases B, BD and L (containing xylanase, a mixture of xylanase and cellulase, and a combination of cellulase, xylanase and polygalacturonase activities, respectively) was assessed. Principal component analysis was used to assess correlations (if any) between the different alkaline solvents, carbohydrases and changing biomass:solvent on extraction efficiency. The highest protein content (56.64 ± 0.43 % (w/w)) was obtained using KOH assisted extraction with carbohydrase BD. The highest protein and biomass yield recoveries (70.65 ± 1.38 and 21.99 ± 0.43 % (w/w), respectively) were obtained on extraction using NaOH assisted with carbohydrases BD. Electrophoretic, amino acid profile and amino acid score analyses displayed differences depending on the alkaline solvent used for protein extraction. Appropriate selection of the alkaline solvent, carbohydrase and biomass:volume combination during protein extraction can contribute to the production of compositionally enhanced high-quality protein concentrates from P. palmata.

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.