lncRNA WAC-AS1通过miR-204-5p/HOXC8轴促进胃癌进展

IF 5 2区医学 Q2 Medicine

Translational Oncology Pub Date : 2024-10-11 DOI:10.1016/j.tranon.2024.102139

Yan Liu , Kaixuan Li , Yongjian Gao , Ye Feng , Xiaoling Zhao , Ruizhi Hou

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引用次数: 0

摘要

LncRNAs影响肿瘤的发生，尽管lncRNAs在胃癌（GC）中的发生、调控和生理机制已有报道，但对lncRNAs的研究仍有很大价值。通过全面的生物信息学分析，我们筛选出了候选lncRNA WAC-AS1（WAC-AS1）。我们利用qRT-PCR技术分析了WAC-AS1在GC相关组织和细胞中的表达。研究了WAC-AS1对GC生长和转移的影响。通过双荧光素酶报告、FISH、RIP和RNA pull-down实验，确定了LncRNA WC-AS-miR-204-5p-HOXC8的相互作用。我们观察到 WAC-AS1 在 GC 细胞和组织中的表达大幅上调。WAC-AS1 通过 miR-204-5p/HOXC8 轴促进了 GC 的增殖、侵袭和迁移。WAC-AS1在促进GC进展方面起着促癌作用。

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lncRNA WAC-AS1 promotes the progression of gastric cancer through miR-204-5p/HOXC8 axis

LncRNAs affect tumorigenesis, and although the genesis, regulation and physiological mechanism of lncRNAs in gastric cancer (GC) have been reported, the research of lncRNAs still have a lot of value. Through comprehensive bioinformatics analysis, we screened the candidate lncRNA WAC-AS1(WAC-AS1). We analyzed WAC-AS1 expression in GC related tissues and cells using qRT-PCR. WAC-AS1’s impact on GC growth and metastasis was investigated. LncRNA WC-AS-miR-204-5p-HOXC8 interaction was established through dual-luciferase reporter, FISH, RIP and RNA pull-down assay. We observed substantial upregulation in WAC-AS1 expression in cells and tissues of GC. WAC-AS1 through miR-204-5p/HOXC8 axis promoted GC proliferation, invasion, and migration. WAC-AS1 plays a cancer-promoting role for promoting the progression of GC.

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来源期刊

Translational Oncology ONCOLOGY-

CiteScore

8.40

自引率

2.00%

发文量

314

审稿时长

54 days

期刊介绍： Translational Oncology publishes the results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of oncology patients. Translational Oncology will publish laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer. Peer reviewed manuscript types include Original Reports, Reviews and Editorials.