Interactions between broiler parent stock age and egg pre-incubation duration: effects on embryo development, hatchability, day-old chick weight, and yolk sac weight

Egg storage is a common practice in commercial hatcheries, but prolonged storage can negatively impact hatchability, causing a significant problem for the poultry industry. Repeated pre-incubation could mitigate the decline depending on the age of the parent stock. The study investigated the interactions between parent stock age (30, 45, and 58 weeks) and repeated pre-incubation on the hatchability and embryo development of 15-day stored eggs from Ross 308 parent stock. Three different pre-incubation durations were employed during storage: no pre-incubation and twice at the 5th and 10th days of egg storage for either 4 or 8 h. For each parent stock age, 3 600 eggs collected on the same day from the same parent stock were used for hatchability assessment. The duration of pre-incubation was determined as the eggshell temperature increased from 28 °C to a maximum of 35 °C and then cooled to 28 °C; the persistency at 35 °C was either 1 or 4 h for the total pre-incubation period. The hatchability of both set and fertile eggs and early, middle, and late embryonic mortality depended on the parent stock’s age (P < 0.001). Pre-incubation alone did not have any impact on hatchability. Conversely, a significant interaction was observed between parent stock age and pre-incubation duration on the hatchability of fertile eggs (P = 0.001). At 30 weeks of parent stock age, both durations of pre-incubation positively influenced the hatchability. At 45 weeks of parent stock age, pre-incubation length had no effect on hatchability. At 58 weeks of parent stock age, a longer pre-incubation period, 2 × 8 h, was associated with decreased hatchability. Early embryonic mortality was not influenced by pre-incubation. However, a significant interaction was observed (P = 0.003). At 30 weeks of parent stock age, both short and long lengths of pre-incubation were associated with a decrease in early embryonic mortality. However, at 45 and 58 weeks, pre-incubation did not significantly affect early embryonic mortality. Additionally, longer pre-incubation periods significantly increased middle mortality compared to untreated eggs (P = 0.035). The median of embryo development for untreated eggs was the same across all ages (stage 10). In the older parent stock (45 and 58 weeks), repeated pre-incubation increased variability in embryo development, while in the younger parent stock, repeated pre-incubation decreased variability in embryo development. In conclusion, the duration and the frequency of pre-incubation should be specified based on the age of the parent stock.