S. S. Kavya, V. Mahantesha, A. Chowdappa, M. Mantesh, P. S. Pooja, V. Venkataravanappa, C. N. Lakshminarayana Reddy
{"title":"番茄褐皱果实病毒与印度番茄果实上的叶片马赛克、斑点和褐皱斑块有关","authors":"S. S. Kavya, V. Mahantesha, A. Chowdappa, M. Mantesh, P. S. Pooja, V. Venkataravanappa, C. N. Lakshminarayana Reddy","doi":"10.1007/s13314-024-00534-5","DOIUrl":null,"url":null,"abstract":"<div><p>Eleven fruit samples from the tomato plants exhibiting different kinds of symptoms on leaves and fruits were collected from farmers fields located in two states (Karnataka and Maharashtra) of India. To confirm the identity of the pathogen, eleven tomato fruit samples were subjected DAS-ELISA, which revealed that all samples showed positive reaction to ToBRFV antibodies. Further, total RNA was isolated from infected fruits and subjected to RT-PCR assay using tomato brown rugose fruit virus (ToBRFV) coat protein gene specific primers. The resulting PCR amplicons of ~ 500 bp from eleven tomato samples were cloned, and sequenced. Sequence analysis of the CP gene from eleven isolates showed nucleotide identity of 100% with several ToBRFV isolates reported from different countries around the world. This result was further confirmed through phylogenetic analysis, wherein the eleven ToBRFV isolates are clustered closely with isolates reported from different parts of the world. To the best of our knowledge, this is the first record of ToBRFV infecting tomato in India.</p></div>","PeriodicalId":45862,"journal":{"name":"Australasian Plant Disease Notes","volume":"19 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tomato brown rugose fruit virus associated with leaf mosaic, mottling and brown rugose patches on fruits of tomato in India\",\"authors\":\"S. S. Kavya, V. Mahantesha, A. Chowdappa, M. Mantesh, P. S. Pooja, V. Venkataravanappa, C. N. Lakshminarayana Reddy\",\"doi\":\"10.1007/s13314-024-00534-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Eleven fruit samples from the tomato plants exhibiting different kinds of symptoms on leaves and fruits were collected from farmers fields located in two states (Karnataka and Maharashtra) of India. To confirm the identity of the pathogen, eleven tomato fruit samples were subjected DAS-ELISA, which revealed that all samples showed positive reaction to ToBRFV antibodies. Further, total RNA was isolated from infected fruits and subjected to RT-PCR assay using tomato brown rugose fruit virus (ToBRFV) coat protein gene specific primers. The resulting PCR amplicons of ~ 500 bp from eleven tomato samples were cloned, and sequenced. Sequence analysis of the CP gene from eleven isolates showed nucleotide identity of 100% with several ToBRFV isolates reported from different countries around the world. This result was further confirmed through phylogenetic analysis, wherein the eleven ToBRFV isolates are clustered closely with isolates reported from different parts of the world. To the best of our knowledge, this is the first record of ToBRFV infecting tomato in India.</p></div>\",\"PeriodicalId\":45862,\"journal\":{\"name\":\"Australasian Plant Disease Notes\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australasian Plant Disease Notes\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13314-024-00534-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australasian Plant Disease Notes","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13314-024-00534-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Tomato brown rugose fruit virus associated with leaf mosaic, mottling and brown rugose patches on fruits of tomato in India
Eleven fruit samples from the tomato plants exhibiting different kinds of symptoms on leaves and fruits were collected from farmers fields located in two states (Karnataka and Maharashtra) of India. To confirm the identity of the pathogen, eleven tomato fruit samples were subjected DAS-ELISA, which revealed that all samples showed positive reaction to ToBRFV antibodies. Further, total RNA was isolated from infected fruits and subjected to RT-PCR assay using tomato brown rugose fruit virus (ToBRFV) coat protein gene specific primers. The resulting PCR amplicons of ~ 500 bp from eleven tomato samples were cloned, and sequenced. Sequence analysis of the CP gene from eleven isolates showed nucleotide identity of 100% with several ToBRFV isolates reported from different countries around the world. This result was further confirmed through phylogenetic analysis, wherein the eleven ToBRFV isolates are clustered closely with isolates reported from different parts of the world. To the best of our knowledge, this is the first record of ToBRFV infecting tomato in India.
期刊介绍：
Australasian Plant Disease Notes is an international journal for publication of short reports from all countries which describe original research that transcends national boundaries. All aspects of plant pathology are covered including new geographical records of diseases or pathogens, new pathogens or pathogen strains, taxonomic papers, quarantine notes, disease management and diagnostic methods. Australasian Plant Disease Notes is a publication of the Australasian Plant Pathology Society.
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