通过盘卷相互作用，用工程细菌孢子对材料进行宏观组装。

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2024-11-15 Epub Date: 2024-10-11 DOI:10.1021/acssynbio.4c00468

Lucas Korbanka, Ju-An Kim, Seunghyun Sim

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引用次数: 0

摘要

在此，我们报告了通过组装工程细菌孢子形成的宏观材料。通过使用 T7 驱动的表达系统改造孢子，使其表面显示出高密度的 pH 响应自结合蛋白。孢子表面的工程表面蛋白可在蛋白质水平上实现 pH 依赖性结合，并能组装成颗粒状材料。机械性能在 pH 值变化时基本保持不变，但侵蚀稳定性与 pH 值相关，这与工程表面蛋白之间的相互作用与 pH 值相关的方式是一致的。我们的发现将合成生物学应用于宏观材料的设计，并揭示了不同长度尺度上线圈相互作用的影响。

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Macroscopic Assembly of Materials with Engineered Bacterial Spores via Coiled-Coil Interaction.

Herein, we report macroscopic materials formed by the assembly of engineered bacterial spores. Spores were engineered by using a T7-driven expression system to display a high density of pH-responsive self-associating proteins on their surface. The engineered surface protein on the spore surface enabled pH-dependent binding at the protein level and enabled the assembly of granular materials. Mechanical properties remained largely constant with changing pH, but erosion stability was pH-dependent in a manner consistent with the pH-dependent interaction between the engineered surface proteins. Our finding utilizes synthetic biology for the design of macroscopic materials and illuminates the impact of coiled-coil interaction across various length scales.

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.