利用微型质谱仪快速识别和现场分析用于鉴定花梨木香味的化学标记。

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2024-09-29 DOI:10.1016/j.jpba.2024.116490

Siyu Zhang , Linnan Li , Yanqiao Xie , Linhong Fan , Yu Wang , Nan Wang , Zhuzhen Han , Zhengtao Wang , Kaixian Chen , Li Yang

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引用次数: 0

摘要

Dalbergia odorifera T. Chen（Dalbergiae Odoriferae Lignum，DOL）因其在传统中药中的治疗应用和在制作奢侈品方面的价值而闻名于世，但由于心材形成过程缓慢以及随后的市场掺假问题而面临挑战。假冒产品的激增需要可靠的现场快速鉴定方法。本研究旨在建立一种快速、绿色和现场鉴定 DOL 真伪的方法。利用配备纸毛细管喷雾（PCS）技术的微型质谱仪（mini MS），对来自不同地点的 10 批 DOL 样品进行了全面分析。采用多元统计方法对样品进行分类，并找出表明真伪的化学标记。随后的 MS 引导分离和薄层色谱法（TLC）验证了标记的有效性，并通过分析生态尺度（AES）、绿色分析程序指数（GAPI）和分析绿色度（AGREE）评估了绿色概况。采用无监督聚类分析法对 PCS-mini MS 检测到的 10 批 DOL 样品进行了真假分类。萨替瓦酮（m/z 301.1，VIP=6.0，p=0.000001）和3'-O-甲基紫罗兰酮（m/z 331.1，VIP=3.2，p=0.000382）被认为是快速鉴定 DOL 的化学标记物。TLC法的结果与该方法一致，且新方法更加绿色环保。应用微型质谱通过特定的化学标记对 DOL 进行现场鉴定具有操作简便、高效、绿色和准确等显著优势。采用这一策略有望促进对 DOL 的有效监管，确保其真实性和质量。

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Rapid identification and on-site analysis by miniature mass spectrometry of chemical markers for fragrant rosewood authentication

Dalbergia odorifera T. Chen (Dalbergiae Odoriferae Lignum, DOL), renowned for its therapeutic applications in traditional Chinese medicine and its value in crafting luxury items, faces challenges due to the slow heartwood formation process and subsequent market adulteration. The proliferation of counterfeit products necessitates reliable methods for rapid, on-site authentication. This study aims to establish a rapid, green, and on-site method to identify the authenticity of DOL. A comprehensive analysis was conducted on 10 batches of DOL samples sourced from diverse locations, utilizing a miniature mass spectrometer (mini MS) equipped with a paper capillary spray (PCS) technique. Multivariate statistical approaches were employed to classify the samples and pinpoint chemical markers indicative of authenticity. Subsequent MS-guided separation and thin-layer chromatography (TLC) verified the markers' validity and assessed the greenness profile by Analytical Eco-scale (AES), Green Analytical Procedure Index (GAPI), and Analytical GREEnness (AGREE). A total of 10 batches of DOL samples detected by PCS-mini MS were classified into authentic and counterfeit, by unsupervised cluster analysis. Sativanone (m/z 301.1, VIP=6.0, p=0.000001) and 3′-O-methylviolanone (m/z 331.1, VIP=3.2, p=0.000382) were regarded as the chemical markers for the rapid identification of DOL. The results of the TLC method were consistent with this method, and the new method is greener. The application of mini MS for on-site authentication of DOL via specific chemical markers offers significant advantages, including operational simplicity, high efficiency, greenness, and accuracy. The deployment of this strategy promises to facilitate the effective regulation of DOL, ensuring authenticity and quality.

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.