E. Malle , J. Nimpf , H.J. Leis , H. Wurm , H. Gleispach , G.M. Kostner
{"title":"血小板聚集过程中的环加氧酶和脂加氧酶代谢物:负离子化学电离-气相色谱/质谱法定量测定","authors":"E. Malle , J. Nimpf , H.J. Leis , H. Wurm , H. Gleispach , G.M. Kostner","doi":"10.1016/0262-1746(87)90059-X","DOIUrl":null,"url":null,"abstract":"<div><p>This study was aimed at investigating systemetically the aggregation of human gel filtered platelets induced by various physiological stimuli such as thrombin (0.25 U/ml), collagen (2 μg/ml), a mixture of thrombin and collagen, and ADP (2–5 μM). For quantitative measurement of TXB2, PGF2a and 12-HETE, negative ion chemical ionization - gas chromatography/mass spectrometry using stable isotope dilution was applied. Stimulation by thrombin, collagen and the combined agonists resulted in an increase of TXB2 (up to 54 ng/1 × 10<sup>8</sup> platelets) and 12-HETE (up to 44 ng/1 × 10<sup>8</sup> platelets) during platelet aggregation. The simulation with ADP showed only little increase of these metabolites and this only during the second wave of aggregation. Very little amounts of PGF2α were produced during thrombin stimulated aggregation.</p></div>","PeriodicalId":20720,"journal":{"name":"Prostaglandins, leukotrienes, and medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1987-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0262-1746(87)90059-X","citationCount":"21","resultStr":"{\"title\":\"Cyclooxygenase and lipoxygenase metabolites during platelet aggregation: Quantitative measurement by negative ion chemical ionization — gas chromatography / mass spectrometry\",\"authors\":\"E. Malle , J. Nimpf , H.J. Leis , H. Wurm , H. Gleispach , G.M. Kostner\",\"doi\":\"10.1016/0262-1746(87)90059-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study was aimed at investigating systemetically the aggregation of human gel filtered platelets induced by various physiological stimuli such as thrombin (0.25 U/ml), collagen (2 μg/ml), a mixture of thrombin and collagen, and ADP (2–5 μM). For quantitative measurement of TXB2, PGF2a and 12-HETE, negative ion chemical ionization - gas chromatography/mass spectrometry using stable isotope dilution was applied. Stimulation by thrombin, collagen and the combined agonists resulted in an increase of TXB2 (up to 54 ng/1 × 10<sup>8</sup> platelets) and 12-HETE (up to 44 ng/1 × 10<sup>8</sup> platelets) during platelet aggregation. The simulation with ADP showed only little increase of these metabolites and this only during the second wave of aggregation. Very little amounts of PGF2α were produced during thrombin stimulated aggregation.</p></div>\",\"PeriodicalId\":20720,\"journal\":{\"name\":\"Prostaglandins, leukotrienes, and medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0262-1746(87)90059-X\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins, leukotrienes, and medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/026217468790059X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins, leukotrienes, and medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/026217468790059X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cyclooxygenase and lipoxygenase metabolites during platelet aggregation: Quantitative measurement by negative ion chemical ionization — gas chromatography / mass spectrometry
This study was aimed at investigating systemetically the aggregation of human gel filtered platelets induced by various physiological stimuli such as thrombin (0.25 U/ml), collagen (2 μg/ml), a mixture of thrombin and collagen, and ADP (2–5 μM). For quantitative measurement of TXB2, PGF2a and 12-HETE, negative ion chemical ionization - gas chromatography/mass spectrometry using stable isotope dilution was applied. Stimulation by thrombin, collagen and the combined agonists resulted in an increase of TXB2 (up to 54 ng/1 × 108 platelets) and 12-HETE (up to 44 ng/1 × 108 platelets) during platelet aggregation. The simulation with ADP showed only little increase of these metabolites and this only during the second wave of aggregation. Very little amounts of PGF2α were produced during thrombin stimulated aggregation.
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