UBE2Q1 作为肺腺癌的新型癌症生物标记物

IF 2.3 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

American Journal of the Medical Sciences Pub Date : 2025-03-01 DOI:10.1016/j.amjms.2024.10.002

Wei Jiang MD , Yuchao Wang MD , Jue Zou MD , Li Li MD , Chunhua Xu MD

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引用次数: 0

摘要

目的：泛素结合酶（E2s）参与多种肿瘤促进过程。UBE2Q1 是 E2 家族的一员。本研究旨在检测 UBE2Q1 在人肺腺癌中的表达水平，并研究其恶性生物学功能：方法：采用 Western 印迹、qRT-PCR 和免疫组化技术检测 UBE2Q1 在人肺腺癌组织中的表达。免疫组化分析了 99 例肺腺癌样本中 UBE2Q1 表达与临床病理变量之间的关联。在体外实验中，建立 UBE2Q1 基因敲除模式，通过 Western 印迹分析凋亡、细胞周期和上皮-间质转化（EMT）标志物。CCK8、集落形成、Transwell和侵袭实验分析了UBE2Q1敲除对肺癌细胞增殖、转移和侵袭的影响：结果：UBE2Q1在肺腺癌中高表达，其表达水平与TNM分期、肿瘤大小和淋巴结转移有关。UBE2Q1 的高表达水平还与生存率低有关，是一个独立的危险因素。体外实验也证实，稳定下调 UBE2Q1 可促进细胞凋亡、诱导 G2/M 细胞周期停滞和调控 EMT。沉默 UBE2Q1 可显著降低肺肿瘤细胞的增殖、迁移和侵袭能力：结论：UBE2Q1 可作为肺腺癌的预后生物标志物和新的治疗靶点。

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UBE2Q1 as a novel cancer biomarker for lung adenocarcinoma

Purpose

Ubiquitin-conjugating enzymes (E2s) participate in various tumor-promoting processes. UBE2Q1 is a member of the E2 family. This research aimed to detect the expression level of UBE2Q1 in human lung adenocarcinoma and to study its malignant biological function.

Methods

Western blot, qRT-PCR and immunohistochemistry was used to measure the expression of UBE2Q1 in human lung adenocarcinoma tissues. The association between UBE2Q1 expression and clinic-pathological variables in 99 lung adenocarcinoma samples was analyzed by immunohistochemistry. In vitro experiment, establishing UBE2Q1 knockdown pattern, the markers of apoptosis, cell cycle and epithelial-mesenchymal transition (EMT) were analyzed by Western blot. CCK8, colony formation, Transwell and invasion assay analyzed the effect of UBE2Q1 knockdown on the proliferation, metastasis and invasion of lung cancer cells.

Results

UBE2Q1 was overexpressed in lung adenocarcinoma, and the expression level of UBE2Q1 was related with TNM stage, tumor size, and lymph node metastasis. The high level of UBE2Q1 expression was also associated with poor survival and was an independent risk factor. In vitro, It was also confirmed that steady downregulation of UBE2Q1 could promote apoptosis, induce G2/M cell cycle arrest and regulate EMT. UBE2Q1 silencing dramatically reduce lung tumor cells proliferation, migration and invasion capacities.

Conclusions

UBE2Q1 may serve as a prognostic biomarker and a new therapeutic target of lung adenocarcinoma.

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来源期刊

American Journal of the Medical Sciences 医学-医学：内科

CiteScore

4.40

自引率

0.00%

发文量

303

审稿时长

1.5 months

期刊介绍： The American Journal of The Medical Sciences (AJMS), founded in 1820, is the 2nd oldest medical journal in the United States. The AJMS is the official journal of the Southern Society for Clinical Investigation (SSCI). The SSCI is dedicated to the advancement of medical research and the exchange of knowledge, information and ideas. Its members are committed to mentoring future generations of medical investigators and promoting careers in academic medicine. The AJMS publishes, on a monthly basis, peer-reviewed articles in the field of internal medicine and its subspecialties, which include: Original clinical and basic science investigations Review articles Online Images in the Medical Sciences Special Features Include: Patient-Centered Focused Reviews History of Medicine The Science of Medical Education.