转录组和代谢组的整合分析揭示了极高海拔地区藏绵羊精子发生的潜在机制。

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2024-10-09 DOI:10.1016/j.ygeno.2024.110949

Miaoshu Zhang , Xuejiao An , Chao Yuan , Tingting Guo , Binpeng Xi , Jianbin Liu , Zengkui Lu

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引用次数: 0

摘要

睾丸在家畜雄性生殖过程中具有不可或缺的功能。许多基因和代谢物与睾丸发育和精子生成有关。然而，人们对与雄性藏羊繁殖力相关的生物调控途径知之甚少。本研究采集了极高海拔的霍巴藏羊（HB，海拔4614米）和岗巴藏羊（GB，海拔4401米）以及中高海拔的高山美利奴羊（AM，海拔2500米，对照组）的睾丸，研究了它们的基因和代谢物水平。利用苏木精-伊红（HE）染色法对藏系绵羊和高山美利奴羊的睾丸组织进行组织学分析，并通过转录组学和代谢组学对其睾丸进行分析，以探索睾丸发育和精子发生的潜在机制。统计结果显示，HB 和 GB 的睾丸曲细精管横截面积、曲细精管直径和生精上皮厚度均显著小于 AM（P＜0.05）。

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Integration analysis of transcriptome and metabolome revealed the potential mechanism of spermatogenesis in Tibetan sheep (Ovis aries) at extreme high altitude

Testis has an indispensable function in male reproduction of domestic animals. Numerous genes and metabolites were related to testicular development and spermatogenesis. However, little is known about the biological regulation pathways associated with fecundity in male Tibetan sheep. In this study, Testes were collected from Huoba Tibetan sheep (HB, 4614 m) and Gangba Tibetan sheep (GB, 4401 m) at extreme high altitude, and Alpine Merino sheep (AM, 2500 m, control group) at medium-high altitude, investigating the genes and metabolites levels of them. The histological analysis of testicular tissue using hematoxylin-eosin (HE) staining was performed for Tibetan sheep and Alpine Merino sheep, and the testes of them were analyzed by transcriptomics and metabolomics to explore the potential mechanism of testicular development and spermatogenesis. The statistical results showed that the cross-sectional area of testicular seminiferous tubules, diameter of seminiferous tubules, and spermatogenic epithelium thickness were significantly smaller in HB and GB than in AM (P < 0.05). Overall, 5648 differentially expressed genes (DEGs) and 336 differential metabolites (DMs) were identified in three sheep breeds, which were significantly enriched in spermatogenesis and other related pathways. According to integrated metabolomic and transcriptomic analysis, glycolysis/gluconeogenesis, AMPK signaling pathway, and TCA cycle, were predicted to have dramatic effects on the spermatogenesis of Tibetan sheep. Several genes (including Wnt2, Rab3a, Sox9, Hspa8, and Slc38a2) and metabolites (including L-histidinol, Glucose, Fumaric acid, Malic acid, and Galactose) were significantly enriched in pathways related to testicular development and spermatogenesis, and might affect the reproduction of Tibetan sheep by regulating the acrosome reaction, meiotic gene expression, and the production of sex hormones. Our results provide further understanding of the key genes and metabolites involved in testicular development and spermatogenesis in Tibetan sheep.

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.