利用新开发的气相沉积系统,ToF-SIMS 研究环境对质谱成像基质涂层中分析物迁移的影响。

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Thorsten Adolphs, Michael Bäumer, Florian Bosse, Bart Jan Ravoo, Richard E Peterson, Heinrich F Arlinghaus, Bonnie J Tyler
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引用次数: 0

摘要

高分辨率质谱图像在组织和单细胞研究等生物应用领域的重要性与日俱增。基质增强二次离子质谱(ME-SIMS)和基质辅助激光解吸/电离(MALDI)是两种很有前途的技术。对于这两种技术,在分析前必须在相关样品上涂覆基质,分析物必须迁移到基质中。人们对这种迁移所涉及的机制以及影响迁移的因素知之甚少,这导致了重现性方面的困难。在这项工作中,我们开发并在公司内部建立了一个带有喷流室和样品冷却装置的升华基质涂层器,用于控制物理气相沉积。在该系统中,镀膜机和质谱仪之间的样品转移无需破坏真空,这为研究环境对分析物迁移的影响提供了便利。利用 3D-SIMS 成像技术研究了暴露在环境空气中对涂有基质 α-氰基-4-羟基肉桂酸(CHCA)的两种分析物(一种脂质和一种肽)迁移的影响。虽然分析物在基质中的分布在真空中储存 21 小时后变化很小,但暴露在环境空气中后观察到分析物有明显的重新分布。脂质的影响程度大于肽。需要进一步研究湿度在重新分布过程中的作用以及分析物重新分布对 MALDI 测量的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ToF-SIMS Investigation of Environmental Effects on Analyte Migration in Matrix Coatings for Mass Spectrometry Imaging Using a Newly Developed Vapor Deposition System.

High resolution mass spectrometry images are of increasing importance in biological applications, such as the study of tissues and single cells. Two promising techniques for this are matrix-enhanced secondary ion mass spectrometry (ME-SIMS) and matrix-assisted laser desorption/ionization (MALDI). For both techniques, the sample of interest must be coated with a matrix prior to analysis, and analytes must migrate into the matrix. The mechanisms involved in this migration and the factors that influence the migration are poorly understood, which lead to difficulties with reproducibility. In this work, a sublimation matrix coater with an effusion cell and sample cooling was developed and built in-house for controlled physical vapor deposition. In this system, sample transfer between the coater and mass spectrometer is possible without breaking vacuum, which facilitates the study of environmental influences on analyte migration. The influence of exposure to ambient air on the migration of two analytes (a lipid and a peptide), which were coated with the matrix α-cyano-4-hydroxycinnamic acid (CHCA), was studied using 3D-SIMS imaging. Although the distribution of analyte in the matrix changed very little after 21 h of storage in vacuum, significant redistribution of the analyte was observed after exposure to ambient air. The magnitude of the effect was greater for the lipid than for the peptide. Further work is needed to determine the role of humidity in the redistribution process and the impact of analyte redistribution on MALDI measurements.

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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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