利用 FIA 和超高效液相色谱-质谱联用仪（Orbitrap）对巴西无刺蜂的（Geo）蜂胶进行代谢组学分析。

IF 3.1 3区医学 Q2 CHEMISTRY, ANALYTICAL

Journal of pharmaceutical and biomedical analysis Pub Date : 2024-10-05 DOI:10.1016/j.jpba.2024.116497

João Fábio Turco , João Benhur Mokochinski , Yohandra Reyes Torres

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引用次数: 0

摘要

巴西本地无刺蜜蜂（Meliponini）制造的（geo）蜂胶主要用于民间医药，特别是土著和前逃亡黑奴社区以及养蜂人家庭，但其药理活性正逐步得到认可。本研究采用流式进样分析法（FIA）和超高效液相色谱法（UHPLC），在高分辨率 Orbitrap 质量分析仪（HRMS）上对 Melipona marginata、M. quadrifasciata、M. scutellaris 和 Tetragonisca angustula 的（地）蜂胶乙醇提取物（EEGs）进行了分析，以研究和比较其化学特征。基于超高效液相色谱-HRMS 实验和生物信息学工具的非靶向代谢组学方法，可注释出 59 种不同类别的化合物，如类黄酮、酚类化合物、糖类、萜类化合物和脂类。此外，利用多元工具和流动注射-高分辨质谱法（FIA-HRMS），可以对样品进行分类，并识别与蜜蜂种类或属以及地理来源有关的标记离子，以此作为概念验证。

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Metabolomics analysis of (Geo)propolis from Brazilian stingless bees by FIA and UHPLC-HRMS (Orbitrap)

Native stingless bees (Meliponini) from Brazil make (geo)propolis which is largely used in folk medicine, specially by indigenous and quilombos communities and beekeepers´ families but are progressively being recognized for their pharmacological activities. In this study, the ethanolic extracts of (geo)propolis (EEGs) from Melipona marginata, M. quadrifasciata, M. scutellaris, and Tetragonisca angustula were analysed by Flow injection analysis (FIA) and Ultra-high performance liquid chromatography (UHPLC) in a high resolution Orbitrap mass analyser (HRMS) to investigate and compare their chemical profile. Untargeted metabolomic approach based on UHPLC-HRMS experiments, and bioinformatic tools, allowed to annotate 59 compounds from diverse classes such as: flavonoids, phenolic compounds, sugars, terpenoids, and lipids. In addition, using multivariate tools and Flow injection- high resolution mass spectrometry (FIA-HRMS), it was possible to classify samples and identify marker ions related to the bee species or genus and to the geographical origin as a proof of concept.

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来源期刊

Journal of pharmaceutical and biomedical analysis 医学-分析化学

CiteScore

6.70

自引率

5.90%

发文量

588

审稿时长

37 days

期刊介绍： This journal is an international medium directed towards the needs of academic, clinical, government and industrial analysis by publishing original research reports and critical reviews on pharmaceutical and biomedical analysis. It covers the interdisciplinary aspects of analysis in the pharmaceutical, biomedical and clinical sciences, including developments in analytical methodology, instrumentation, computation and interpretation. Submissions on novel applications focusing on drug purity and stability studies, pharmacokinetics, therapeutic monitoring, metabolic profiling; drug-related aspects of analytical biochemistry and forensic toxicology; quality assurance in the pharmaceutical industry are also welcome. Studies from areas of well established and poorly selective methods, such as UV-VIS spectrophotometry (including derivative and multi-wavelength measurements), basic electroanalytical (potentiometric, polarographic and voltammetric) methods, fluorimetry, flow-injection analysis, etc. are accepted for publication in exceptional cases only, if a unique and substantial advantage over presently known systems is demonstrated. The same applies to the assay of simple drug formulations by any kind of methods and the determination of drugs in biological samples based merely on spiked samples. Drug purity/stability studies should contain information on the structure elucidation of the impurities/degradants.