高渗条件对果蝇幼虫冷却细胞的影响

IF 4.2 3区 医学 Q2 NEUROSCIENCES
Frontiers in Cellular Neuroscience Pub Date : 2024-09-23 eCollection Date: 2024-01-01 DOI:10.3389/fncel.2024.1347460
Hua Bai, Trisha Naidu, James B Anderson, Hector Montemayor, Camie Do, Lina Ni
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引用次数: 0

摘要

黑腹果蝇表现出多种高度复杂的温度感应系统,使其能够有效应对温度变化并进行导航。先前的研究发现,果蝇幼虫体内有三种背器官冷却细胞(DOCCs),由两个A型细胞和一个B型细胞组成,它们具有不同的钙动力学特性。在高渗条件下,钙成像显示 A 型 DOCC 对低温保持反应。与此相反,一部分 B 型 DOCC 不显示可检测到的 GCaMP 基线信号,其余可检测到的 B 型 DOCC 显示出较低的温度反应。A 型和 B 型 DOCCs 的激活依赖于离子受体(IR)家族的相同成员:IR21a、IR93a 和 IR25a。与 B 型 DOCCs 相比,A 型 DOCCs 体外的 IR93a 水平更高。在高渗环境中,过表达Ir93a能恢复B型细胞对低温的钙离子反应,但不能恢复能检测到GCaMP基线的B型细胞的比例,这表明IR93a在维持高渗条件下的温度反应方面起着选择性作用。我们的研究结果发现了 B 型 DOCC 在整合温度和强直刺激方面的新功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The impacts of hypertonic conditions on Drosophila larval cool cells.

Drosophila melanogaster exhibits multiple highly sophisticated temperature-sensing systems, enabling its effective response and navigation to temperature changes. Previous research has identified three dorsal organ cool cells (DOCCs) in fly larvae, consisting of two A-type and one B-type cell with distinct calcium dynamics. When subjected to hypertonic conditions, calcium imaging shows that A-type DOCCs maintain their responses to cool temperatures. In contrast, a subset of B-type DOCCs does not exhibit detectable GCaMP baseline signals, and the remaining detectable B-type DOCCs exhibit reduced temperature responses. The activation of both A-type and B-type DOCCs depends on the same members of the ionotropic receptor (IR) family: IR21a, IR93a, and IR25a. A-type DOCCs exhibit a higher somal level of IR93a than B-type DOCCs. Overexpression of Ir93a restores B-type calcium responses to cool temperatures, but not the proportion of B-type cells with a detectable GCaMP baseline, in a hypertonic environment, suggesting a selective role of IR93a in maintaining the temperature responses under hypertonic conditions. Our findings identify a novel function of B-type DOCCs in integrating temperature and tonic stimuli.

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来源期刊
CiteScore
7.90
自引率
3.80%
发文量
627
审稿时长
6-12 weeks
期刊介绍: Frontiers in Cellular Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the cellular mechanisms underlying cell function in the nervous system across all species. Specialty Chief Editors Egidio D‘Angelo at the University of Pavia and Christian Hansel at the University of Chicago are supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
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