Yixuan Liao, Raymond G Cavalcante, Jonathan B Waller, Furong Deng, Anne M Scruggs, Yvonne J Huang, Ulus Atasoy, Yahong Chen, Steven K Huang
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Differential methylation was compared among subjects with varying airway inflammation and severity, as measured by fraction of exhaled nitric oxide, forced expiratory volume in one second (FEV1), and Asthma Control Test (ACT) scores.</p><p><strong>Results: </strong>Significant differences in DNA methylation were noted among subjects with different degrees of airway inflammation and asthma severity. These differences in DNA methylation were annotated to genes that were enriched in pathways related to asthma or T cell function and included gene ontology categories related to MHC class II assembly, T cell activation, interleukin (IL)-1, and IL-12. Genes related to P450 drug metabolism, glutathione metabolism, and developmental pathways were also differentially methylated in comparisons between subjects with high vs low FEV1 and ACT. Notable genes that were differentially methylated based on asthma severity included RUNX3, several members of the HLA family, AGT, PTPRC, PTPRJ, and several genes downstream of the JAK2 and TNF signaling pathway.</p><p><strong>Conclusion: </strong>These findings demonstrate how adults with asthma of varying severity possess differences in peripheral blood T cell DNA methylation that contribute to differences in clinical indices of asthma.</p>","PeriodicalId":10366,"journal":{"name":"Clinical Epigenetics","volume":"16 1","pages":"139"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459694/pdf/","citationCount":"0","resultStr":"{\"title\":\"Differences in the DNA methylome of T cells in adults with asthma of varying severity.\",\"authors\":\"Yixuan Liao, Raymond G Cavalcante, Jonathan B Waller, Furong Deng, Anne M Scruggs, Yvonne J Huang, Ulus Atasoy, Yahong Chen, Steven K Huang\",\"doi\":\"10.1186/s13148-024-01750-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>DNA methylation plays a critical role in asthma development, but differences in DNA methylation among adults with varying asthma severity are less well-defined.</p><p><strong>Objective: </strong>To examine how DNA methylomic patterns differ among adults with asthma based on asthma severity and airway inflammation.</p><p><strong>Methods: </strong>Peripheral blood T cells from 35 adults with asthma in Beijing, China, were serially collected over time (130 samples total) and analyzed for global DNA methylation using the Illumina MethylationEPIC Array. 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引用次数: 0
摘要
背景:DNA 甲基化在哮喘的发生发展中起着至关重要的作用,但不同严重程度的成人哮喘患者的 DNA 甲基化差异尚不十分明确:目的:根据哮喘严重程度和气道炎症,研究哮喘成人的 DNA 甲基化模式有何不同:方法:连续采集中国北京 35 名成人哮喘患者的外周血 T 细胞(共 130 个样本),并使用 Illumina MethylationEPIC 阵列分析全局 DNA 甲基化。比较了不同气道炎症和严重程度的受试者之间的甲基化差异,以呼气一氧化氮分数、一秒钟用力呼气容积(FEV1)和哮喘控制测试(ACT)评分来衡量:结果:气道炎症和哮喘严重程度不同的受试者的 DNA 甲基化存在显著差异。这些DNA甲基化差异被注释为富集在与哮喘或T细胞功能相关通路中的基因,包括与MHC II类组装、T细胞活化、白细胞介素(IL)-1和IL-12相关的基因本体类别。与 P450 药物代谢、谷胱甘肽代谢和发育途径相关的基因在 FEV1 和 ACT 值高与低的受试者之间也存在甲基化差异。根据哮喘严重程度发生不同甲基化的基因包括 RUNX3、HLA 家族的几个成员、AGT、PTPRC、PTPRJ 以及 JAK2 和 TNF 信号通路下游的几个基因:这些研究结果表明,不同严重程度的成人哮喘患者的外周血 T 细胞 DNA 甲基化存在差异,而这种差异会导致哮喘临床指标的不同。
Differences in the DNA methylome of T cells in adults with asthma of varying severity.
Background: DNA methylation plays a critical role in asthma development, but differences in DNA methylation among adults with varying asthma severity are less well-defined.
Objective: To examine how DNA methylomic patterns differ among adults with asthma based on asthma severity and airway inflammation.
Methods: Peripheral blood T cells from 35 adults with asthma in Beijing, China, were serially collected over time (130 samples total) and analyzed for global DNA methylation using the Illumina MethylationEPIC Array. Differential methylation was compared among subjects with varying airway inflammation and severity, as measured by fraction of exhaled nitric oxide, forced expiratory volume in one second (FEV1), and Asthma Control Test (ACT) scores.
Results: Significant differences in DNA methylation were noted among subjects with different degrees of airway inflammation and asthma severity. These differences in DNA methylation were annotated to genes that were enriched in pathways related to asthma or T cell function and included gene ontology categories related to MHC class II assembly, T cell activation, interleukin (IL)-1, and IL-12. Genes related to P450 drug metabolism, glutathione metabolism, and developmental pathways were also differentially methylated in comparisons between subjects with high vs low FEV1 and ACT. Notable genes that were differentially methylated based on asthma severity included RUNX3, several members of the HLA family, AGT, PTPRC, PTPRJ, and several genes downstream of the JAK2 and TNF signaling pathway.
Conclusion: These findings demonstrate how adults with asthma of varying severity possess differences in peripheral blood T cell DNA methylation that contribute to differences in clinical indices of asthma.
期刊介绍:
Clinical Epigenetics, the official journal of the Clinical Epigenetics Society, is an open access, peer-reviewed journal that encompasses all aspects of epigenetic principles and mechanisms in relation to human disease, diagnosis and therapy. Clinical trials and research in disease model organisms are particularly welcome.