评估聚焦还原中和试验和酶联免疫吸附试验与斑块还原中和试验在检测麻疹病毒抗体方面的比较。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-05 DOI:10.1016/j.biologicals.2024.101795

Somayeh Yaghoobizad , Zahra Norouzbabaei , Nazanin Zahra Shafiei Jandaghi , Abbas Rahimi Foroushani , Kaveh Sadeghi , Shahrokh Izadi , Ghazal Sadat Fatemi-Nasab , Elaheh Heidari , Vahid Salimi , Talat Mokhtari-Azad

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引用次数: 0

摘要

麻疹是由麻疹病毒（MeV）引起的传染性发热疾病。尽管有了有效的疫苗，但在全球范围内消除麻疹仍是当务之急，并且仍然面临挑战。为了估计社区对麻疹的防护能力，需要用灵敏的测试来确定麻疹特异性抗体。酶联免疫吸附试验（ELISA）是评估免疫力的标准检测方法，但可能无法检测出接种疫苗人群的弱抗体反应。斑块缩小中和试验（PRNT）是评估保护性抗体水平的金标准试验，但不适合常规使用。本研究验证了聚焦还原中和试验（FRNT）作为替代方法的有效性。在八次检测中，使用 PRNT、FRNT 和 ELISA 对 50 份血清样本进行了一式三份的分析。数据分析显示，PRNT 法检测出 38 份阳性样本，FRNT 法检测出 37 份阳性样本，ELISA 法检测出 19 份阳性样本。结果表明，ELISA 的灵敏度不足以识别低水平的抗麻疹抗体，而且与中和检测的一致性较弱。相比之下，两种中和检测法具有完美的相关性和相似的灵敏度。FRNT 似乎是 PRNT 的合适替代品，可用于鉴定免疫反应和疫苗接种效果。我们的研究结果凸显了在消灭阶段通过中和方法验证阴性和不明确的 ELISA 结果的必要性。

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Evaluation of the focus reduction neutralization and ELISA tests compared to the plaque reduction neutralization test for the detection of antibodies against measles virus

Measles is an infectious febrile sickness caused by the measles virus (MeV). Despite an effective vaccine, regional elimination of measles remains a global priority and still faces challenges.

To estimate community protection against measles, sensitive tests are needed to identify measles-specific antibodies. The enzyme-linked immunosorbent assay (ELISA) is the standard test for assessing immunity but may fail to detect weak antibody responses in vaccinated populations. The plaque reduction neutralization test (PRNT), is the gold standard test for the assessment of protective antibody levels, however, it is not suitable for routine use. This study validated the focus reduction neutralization test (FRNT) as an alternative.

In eight assay runs, fifty serum samples were analyzed in triplicate using PRNT, FRNT, and ELISA. Data analysis revealed that 38 samples were positive by PRNT, 37 by FRNT, and 19 by ELISA. The results showed that ELISA was not sensitive enough to identify low levels of anti-measles antibodies and showed weak agreement with neutralization assays. In contrast, the two neutralization assays had a perfect correlation and similar sensitivity. FRNT appears to be a suitable alternative to PRNT for characterizing immunological responses and vaccination efficacy.

Our results highlight the necessity of validating negative and equivocal ELISA results through neutralization methods, during the elimination phases.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.