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引用次数: 0
摘要
合成生物学又称工程生物学，是一个将工程学原理应用于生物系统的跨学科领域。改造生物系统的方法之一是修改其 DNA。常见的工作流程包括通过合成创建新的 DNA 部件，然后通过组装将其与其他部件结合使用。MoClo、Phytobricks 和 Loop 等组装标准都基于 Golden Gate，并提供了组合部件的框架。合成生物学开放语言（Synthetic Biology Open Language，SBOL）采用最佳实践来表示构建计划，通过白板设计与其他从业人员交流，并以机器可读格式与实验室自动化工具交流。在此，我们介绍一种软件工具，用于创建 SBOL 构建计划表示法，以模拟 IIS 介导的组装反应并存储相关元数据。
Standardized Golden Gate Assembly Metadata Representation Using SBOL.
Synthetic biology, also known as engineering biology, is an interdisciplinary field that applies engineering principles to biological systems. One way to engineer biological systems is by modifying their DNA. A common workflow involves creating new DNA parts through synthesis and then using them in combination with other parts through assembly. Assembly standards such as MoClo, Phytobricks, and Loop are based on Golden Gate, and provide a framework for combining parts. The Synthetic Biology Open Language (SBOL) has implemented a best practice for representing build plans to communicate them to other practitioners through whiteboard designs and in a machine-readable format for communication with lab automation tools. Here we present a software tool for creating SBOL representations of build plans to simulate type IIS-mediated assembly reactions and store relevant metadata.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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