食物过敏原、噬菌体、细菌和肠道-皮肤轴的真核宿主细胞之间错综复杂的相互影响。

IF 2.5 3区 工程技术 Q2 BIOLOGY
Yale Journal of Biology and Medicine Pub Date : 2024-09-30 eCollection Date: 2024-09-01
Darab Ghadimi, Regina Fölster-Holst, Sophia Blömer, Michael Ebsen, Christoph Röcken, Jumpei Uchiyama, Shigenobu Matsuzaki, Wilhelm Bockelmann
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引用次数: 0

摘要

细菌和食物过敏原通过肠道-皮肤轴与免疫介导的食物过敏有关。然而,还没有数据表明噬菌体有可能用于挽救这一病理过程。我们建立了一个包含结肠细胞(T84 细胞)、巨噬细胞(THP-1 细胞)和肝细胞(Huh7 细胞)的人类三重细胞共培养模型,并在杜氏改良老鹰培养基(DMEM)、DMEM+ 卵清蛋白(OVA)或 DMEM+β-酪蛋白培养基中,在没有或有 KPP22 噬菌体的情况下用铜绿假单胞菌 PAO1(P.a PAO1)进行感染。细胞的生理健康状况通过评估细胞存活率和跨 T84 单层的跨上皮电阻(TEER)来验证。通过测定 IL-1β、IL-8、IL-22 和 IL-25 的分泌,研究了细胞的免疫反应。评估了 P.a PAO1 黏附和侵入 T84 细胞的能力。添加 OVA 或 β-酪蛋白可增强 P.a PAO1 诱导的细胞因子分泌。与单独 PAO1 组和 PAO1+β-casein 组相比,P.a PAO1+OVA 组 T84 单层细胞的存活率和 TEER 更低。OVA 和 β-酪蛋白能显著增加 P.a PAO1 对 T84 细胞的粘附和侵袭。在有 KPP22 噬菌体存在的情况下,这些破坏作用被消除。这些结果表明噬菌体在 PAO1、OVA 和 β-酪蛋白衍生的炎症中起干预作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intricate Crosstalk Between Food Allergens, Phages, Bacteria, and Eukaryotic Host Cells of the Gut-skin Axis.

Bacterial and food allergens are associated with immune-mediated food allergies via the gut-skin axis. However, there has been no data on the potential use of phages to rescue this pathological process. A human triple cell co-culture model incorporating colonocytes (T84 cells), macrophages (THP-1 cells), and hepatocytes (Huh7 cells) was established and infected with Pseudomonas aeruginosa PAO1 (P.a PAO1) in the absence or presence of its KPP22 phage in Dulbecco's Modified Eagle's Medium (DMEM), DMEM+ ovalbumin (OVA), or DMEM+β-casein media. The physiological health of cells was verified by assessing cell viability and Transepithelial electrical resistance (TEER) across the T84 monolayer. The immune response of cells was investigated by determining the secretions of IL-1β, IL-8, IL-22, and IL-25. The ability of P.a PAO1 to adhere to and invade T84 cells was evaluated. The addition of either OVA or β-casein potentiated the P.a PAO1-elicited secretion of cytokines. The viability and TEER of the T84 monolayer were lower in the P.a PAO1+OVA group compared to the P.a PAO1 alone and PAO1+β-casein groups. OVA and β-casein significantly increased the adherence and invasion of P.a PAO1 to T84 cells. In the presence of the KPP22 phage, these disruptive effects were abolished. These results imply that: (1) food allergens and bacterial toxic effector molecules exacerbate each other's disruptive effects; (2) food allergen and bacterial signaling at the gut-skin mucosal surface axis depend on a network of bacteria-phage-eukaryotic host interactions; and (3) phages are complementary for the evaluation of pathobiological processes that occur at the interface between bacteria, host cellular milieu, and food antigens because phages intervene in P.a PAO1-, OVA-, and β-casein-derived inflammation.

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来源期刊
Yale Journal of Biology and Medicine
Yale Journal of Biology and Medicine Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
5.00
自引率
0.00%
发文量
41
期刊介绍: The Yale Journal of Biology and Medicine (YJBM) is a graduate and medical student-run, peer-reviewed, open-access journal dedicated to the publication of original research articles, scientific reviews, articles on medical history, personal perspectives on medicine, policy analyses, case reports, and symposia related to biomedical matters. YJBM is published quarterly and aims to publish articles of interest to both physicians and scientists. YJBM is and has been an internationally distributed journal with a long history of landmark articles. Our contributors feature a notable list of philosophers, statesmen, scientists, and physicians, including Ernst Cassirer, Harvey Cushing, Rene Dubos, Edward Kennedy, Donald Seldin, and Jack Strominger. Our Editorial Board consists of students and faculty members from Yale School of Medicine and Yale University Graduate School of Arts & Sciences. All manuscripts submitted to YJBM are first evaluated on the basis of scientific quality, originality, appropriateness, contribution to the field, and style. Suitable manuscripts are then subject to rigorous, fair, and rapid peer review.
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