肠道炎症与营养补充:益生菌、维生素和草药提取物固定组合在肠道上皮屏障功能障碍体外模型中的作用。

IF 2.5 3区 工程技术 Q2 BIOLOGY
Yale Journal of Biology and Medicine Pub Date : 2024-09-30 eCollection Date: 2024-09-01 DOI:10.59249/JOMF5336
Mattia Tinazzi, Andrea Sacilotto, Veronica Cocetta, Isabella Giacomini, Floriana Raso, Giorgia Bulferi, Heide De Togni, Roberta Lanza, Pierluigi Consolo, Massimiliano Berretta, Monica Montopoli
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引用次数: 0

摘要

肠道微生物群是影响个体健康状况的一个非常重要的因素,它的改变意味着 "菌群失调",而 "菌群失调 "可能与功能性胃肠道疾病和病理状况有关,如炎症性肠病(IBD)、肠易激综合征(IBS)、溃疡性结肠炎(UC)和克罗恩病(CD)以及结肠直肠癌(CRC)。在这项工作中,我们研究了一种名为 ENTERO-AD 的食品补充剂对肠道炎症的影响,该补充剂含有混合益生菌(嗜酸乳杆菌 LA1、罗伊氏杆菌 LR92、双歧杆菌 Bbr8)、母菊和 B 族维生素(B1、B2、B6)。研究使用的体外模型是 Caco-2 细胞,这是一种从人类肠腺癌中提取的培养物;炎症条件是通过处理脂多糖(LPS)和肿瘤坏死因子α/干扰素γ(TNF-α/IFN-γ)[1,2]来实现的。ENTERO-AD的作用通过细胞活力、跨上皮电阻(TEER)测量、细胞旁通透性和免疫荧光进行评估。研究结果表明,ENTERO-AD 对炎症条件下的 Caco-2 细胞有良好的作用。它能改善 Occludin 和 Zonula Occludens-1 (ZO-1) 蛋白的完整性,从而改善 TEER 值并降低细胞旁通透性。这些证据强调了 ENTERO-AD 及其成分在肠道炎症中的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bowel Inflammation and Nutrient Supplementation: Effects of a Fixed Combination of Probiotics, Vitamins, and Herbal Extracts in an In Vitro Model of Intestinal Epithelial Barrier Dysfunction.

The gut microbiota is a very important factor in the state of health of an individual, its alteration implies a situation of "dysbiosis," which can be connected to functional gastrointestinal disorders and pathological conditions, such as Inflammatory Bowel Disease (IBD), Irritable Bowel Syndrome (IBS), Ulcerative Colitis (UC) and Crohn's Disease (CD), and Colorectal Cancer (CRC). In this work, we studied the effect of a food supplement called ENTERO-AD containing a mix of probiotics (Lactobacillus acidophilus LA1, L. reuteri LR92, Bifidobacterium breve Bbr8), Matricaria Chamomilla, and B group vitamins (B1, B2, B6) on intestinal inflammation. The in vitro model used for the study is the Caco-2 cell, a culture derived from human intestinal adenocarcinoma; the inflammatory condition was achieved with treatment with Lipopolysaccharide (LPS) and the association between Tumor necrosis factor α/Interferon γ (TNF-α/IFN-γ) [1,2]. The effect of ENTERO-AD was evaluated by cell viability, measures of Transepithelial Electrical Resistance (TEER), paracellular permeability, and immunofluorescence. Results of the study have shown that ENTERO-AD has a favorable effect on Caco-2 cells in inflammatory conditions. It improves the integrity of Occludin and Zonula Occludens-1 (ZO-1) proteins, leading to an improvement in terms of TEER values and a reduction of paracellular permeability. This evidence underlines the protective effect of ENTERO-AD and its components in intestinal inflammation.

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来源期刊
Yale Journal of Biology and Medicine
Yale Journal of Biology and Medicine Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology
CiteScore
5.00
自引率
0.00%
发文量
41
期刊介绍: The Yale Journal of Biology and Medicine (YJBM) is a graduate and medical student-run, peer-reviewed, open-access journal dedicated to the publication of original research articles, scientific reviews, articles on medical history, personal perspectives on medicine, policy analyses, case reports, and symposia related to biomedical matters. YJBM is published quarterly and aims to publish articles of interest to both physicians and scientists. YJBM is and has been an internationally distributed journal with a long history of landmark articles. Our contributors feature a notable list of philosophers, statesmen, scientists, and physicians, including Ernst Cassirer, Harvey Cushing, Rene Dubos, Edward Kennedy, Donald Seldin, and Jack Strominger. Our Editorial Board consists of students and faculty members from Yale School of Medicine and Yale University Graduate School of Arts & Sciences. All manuscripts submitted to YJBM are first evaluated on the basis of scientific quality, originality, appropriateness, contribution to the field, and style. Suitable manuscripts are then subject to rigorous, fair, and rapid peer review.
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