评估雄性小鼠在触摸屏 5 选 1 连续反应时间任务中反应的稳定性:关注过早反应

IF 4.2 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Arya Rahbarnia, Andrew R Abela, Paul J Fletcher
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引用次数: 0

摘要

五选一连续反应时间任务(5CSRTT)是一种注意力测试,通过过早反应来测量冲动行为,是一种经过充分验证的辅助测量方法。该任务已被改编用于小鼠的触摸屏操作箱,这被认为能提高测试的重复可靠性。很少有研究对这一版本任务中的表现(包括过早反应)的长期稳定性进行评估。我们使用触摸屏 5CSRTT 对重复行为和药理操作后过早反应的稳定性进行了纵向测试。雄性 C57BL/6J 小鼠接受了基线版本的 5CSRTT 训练。然后对它们进行不同版本的任务测试,在测试过程中缩短刺激持续时间,拉长或改变测试间隔。随后,在使用已知会双向影响注意力和冲动行为的药剂--可卡因、阿托西汀和育亨宾后,对过早反应进行了测试。最后,小鼠在 6 个月后接受了拉长试验间隔的 5CSRTT 重新测试。缩短刺激持续时间会影响注意力,降低准确性并增加遗漏,但对过早反应没有影响。拉长试验间隔和改变试验间隔都会增加过早反应。6 个月后,小鼠表现出类似且稳定的过早反应增加水平。可卡因会增加过早反应,但比以前在大鼠中报道的要少。阿托莫西汀可减少过早反应。育亨宾对基线任务中的过早反应没有影响,但在使用拉长的试验间隔进行测试时,过早反应会减少。总之,这些结果突出表明,5CSRTT的触摸屏适应性是纵向测试注意力和冲动行为的一种有效方法,并且对反复药理和行为挑战引起的表现变化保持敏感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessing the stability of responding of male mice in the touchscreen 5 choice serial reaction time task: Focus on premature responding.

The five-choice serial reaction time task (5CSRTT) is a test of attention that provides a well-validated ancillary measure of impulsive action, measured by premature responses. The task has been adapted for mice in touchscreen operant boxes, which is thought to offer improved test-retest reliability. Few studies have assessed the long-term stability of performance, including premature responding in this version of the task. We used the touchscreen 5CSRTT to conduct longitudinal testing of stability of premature responding following repeated behavioral and pharmacological manipulations. Male C57BL/6J mice were trained on a baseline version of the 5CSRTT. They were then tested on versions of the task in which the stimulus duration was reduced, and inter-trial intervals were elongated or varied within-session. Premature responding was subsequently tested following administration of pharmacological agents known to bi-directionally affect attention and impulsive action-cocaine, atomoxetine, and yohimbine. Mice were lastly re-tested 6 months later using the 5CSRTT with elongated inter-trial intervals. A reduced stimulus duration impacted attention, with reduced accuracy and increased omissions, but had no effect on premature responding. Both elongating and varying the inter-trial interval within-session increased premature responses. Mice showed similar and stable levels of increased premature responding 6 months later. Cocaine increased premature responding, though less than previously reported in rats. Atomoxetine reduced premature responding. Yohimbine had no effect on premature responding in the baseline task but decreased premature responding when tested using an elongated inter-trial interval. Overall, these results highlight that the touch screen adaptation of the 5CSRTT is an effective method for longitudinal testing of attention and impulsive action and remains sensitive to performance changes arising from repeated pharmacological and behavioral challenges.

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来源期刊
Journal of Neurochemistry
Journal of Neurochemistry 医学-神经科学
CiteScore
9.30
自引率
2.10%
发文量
181
审稿时长
2.2 months
期刊介绍: Journal of Neurochemistry focuses on molecular, cellular and biochemical aspects of the nervous system, the pathogenesis of neurological disorders and the development of disease specific biomarkers. It is devoted to the prompt publication of original findings of the highest scientific priority and value that provide novel mechanistic insights, represent a clear advance over previous studies and have the potential to generate exciting future research.
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