基于撞击的呼气采样用于药物监测:一项前瞻性试点研究(DrugXhale)。

IF 3.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS
Lutea A A de Jong, Erik J H Olyslager, Jan A Wieferink, Maarten Keizer, Tobias Cornielje, Robbert P Zuidema
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引用次数: 0

摘要

几十年来,以尿液为基质的药物摄入监测一直是戒毒治疗中心的黄金标准。正确进行尿液药物检测可以确定近期使用处方药、非处方药和违禁药物的情况。然而,掺假、替代和隐私等问题促使人们寻找替代基质。这项前瞻性试点研究评估了基于撞击的呼吸采样设备 Breath Explor® 的使用情况,以替代传统的尿液药物监测。呼气样本采用经过验证的 32 组分液相色谱-串联质谱法(LC-MSMS)进行分析。回收率数据表示从呼气装置中提取分析物的效率。对 Breath Explor® 设备进行自动和手动处理后,32 种分析物的平均回收率从 39.5% 到 55.4% 不等。尽管受试者人数较少,但事实证明呼气分析是一种方便易用的方法。总体卡帕值为 0.5,表明与尿液分析的一致性达到中等水平,突出了其作为辅助诊断工具的潜力。所有参与者的呼气样本中美沙酮检测结果均呈阳性(70%为美沙酮,100%为乙二胺四乙酸二丁酯),而相当一部分(90%)参与者的 6-单乙酰吗啡(6-MAM)检测结果呈阳性。这种创新方法具有多项优势,包括无创、降低掺假风险以及能够重复进行自动采样和确认测试。这些研究结果表明,在临床实践中,基于呼气的物质监测可以补充甚至取代传统的基于尿液的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impaction-based exhaled breath sampling for substance monitoring: a prospective pilot study (Drugxhale).

For decades, intake monitoring of drugs using urine as the matrix of choice is the gold standard in drug treatment centers. A properly conducted urine drug test can identify recent use of prescribed, non-prescribed and illicit drugs. However, issues like adulteration, substitution and privacy issues have driven the search for alternative matrices. This prospective pilot study evaluates the use of an impaction-based breath sampling device, Breath Explor®, as an alternative to traditional urine-based drug monitoring. Breath samples were analyzed using a validated 32-component liquid chromatography-tandem mass spectrometry method. Recovery data represent the efficiency of extracting the analytes from the breath devices. Both automated and manual processing of the Breath Explor® devices showed mean recovery rates ranging from 39.5% to 55.4% for the 32 analytes. Despite the small number of subjects, breath analysis proved to be a convenient and easy-to-use methodology. An overall kappa-values of 0.5 indicated a moderate level of agreement with urine analysis, underscoring its potential as a complementary diagnostic tool. All participants tested positive in their breath sample for methadone (70% methadone and 100% EDDP), while a significant portion (90%) tested positive for 6-monoacetylmorphine. This innovative approach offers several advantages, including non-invasiveness, reduced risk of adulteration, and the ability to perfom repeated automated sampling and confirmation testing. These findings suggest that breath-based substance monitoring could complement or even replace traditional urine-based methods in clinical practice.

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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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