Chantal Donovan, Andrew E Thorpe, Rochelle Yarak, Madison Coward-Smith, Amber L Pillar, Henry M Gomez, Min Feng, Xu Bai, Meng Wang, Dia Xenaki, Jay C Horvat, Hui Chen, Brian G G Oliver, Richard Y Kim
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Male adult offspring were then infected with mouse-adapted influenza A virus (A/PR/8/34 H1N1; Flu) and lung and bone marrow immune cell responses were assessed 7 days postinfection. Maternal thirdhand exposure to low-power (<sub>M</sub>LP) or high-power (<sub>M</sub>HP) e-vapor with nicotine (<sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC, respectively) increased the percentage of lung immune cells and neutrophils in the bone marrow. Interestingly, Flu-infected offspring from <sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC groups had lower percentages of lung alveolar macrophages and more pronounced increases in neutrophils in the bone marrow, when compared with offspring from <sub>M</sub>Sham Flu controls. Flu infection also decreased the percentage of lung CD4+ T cells and increased the percentage of lung CD8+ T cells, irrespective of maternal exposure (<sub>M</sub>LP -/+ NIC and <sub>M</sub>HP -/+ NIC). Significantly, both <sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC resulted in blunted activation of lung CD4+ T cells, but only <sub>M</sub>LP + NIC caused blunted activation of lung CD8+ T cells. Together, we show for the first time that maternal thirdhand exposure to e-vapor results in significant, long-lived effects on lung and bone marrow immune cell responses in offspring at baseline and response to Flu infection.<b>NEW & NOTEWORTHY</b> Maternal exposure to environmental residues of e-cigarette use has significant effects on immune cell responses in the lungs and bone marrow of offspring at both baseline and in response to influenza A virus (Flu) infection.</p>","PeriodicalId":7593,"journal":{"name":"American journal of physiology. 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Using two different e-cigarette settings (low vs. high power), BALB/c mice were subjected to thirdhand e-vapor (e-vapor deposited onto towels, towels changed daily) in the absence or presence of nicotine, before, during, and after pregnancy. Male adult offspring were then infected with mouse-adapted influenza A virus (A/PR/8/34 H1N1; Flu) and lung and bone marrow immune cell responses were assessed 7 days postinfection. Maternal thirdhand exposure to low-power (<sub>M</sub>LP) or high-power (<sub>M</sub>HP) e-vapor with nicotine (<sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC, respectively) increased the percentage of lung immune cells and neutrophils in the bone marrow. Interestingly, Flu-infected offspring from <sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC groups had lower percentages of lung alveolar macrophages and more pronounced increases in neutrophils in the bone marrow, when compared with offspring from <sub>M</sub>Sham Flu controls. Flu infection also decreased the percentage of lung CD4+ T cells and increased the percentage of lung CD8+ T cells, irrespective of maternal exposure (<sub>M</sub>LP -/+ NIC and <sub>M</sub>HP -/+ NIC). Significantly, both <sub>M</sub>LP + NIC and <sub>M</sub>HP + NIC resulted in blunted activation of lung CD4+ T cells, but only <sub>M</sub>LP + NIC caused blunted activation of lung CD8+ T cells. Together, we show for the first time that maternal thirdhand exposure to e-vapor results in significant, long-lived effects on lung and bone marrow immune cell responses in offspring at baseline and response to Flu infection.<b>NEW & NOTEWORTHY</b> Maternal exposure to environmental residues of e-cigarette use has significant effects on immune cell responses in the lungs and bone marrow of offspring at both baseline and in response to influenza A virus (Flu) infection.</p>\",\"PeriodicalId\":7593,\"journal\":{\"name\":\"American journal of physiology. 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Lung cellular and molecular physiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1152/ajplung.00078.2024","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/24 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
越来越多的证据表明，第三次接触电子烟蒸汽（电子蒸汽）会对肺部产生有害影响。然而，母体在怀孕期间接触电子烟是否会导致后代发生有害变化尚不清楚。使用两种不同的电子烟设置（低功率和高功率），在没有尼古丁或尼古丁存在的情况下，BALB/c小鼠在妊娠前、妊娠期间和妊娠后都会受到电子烟蒸汽（电子烟蒸汽沉积在毛巾上，毛巾每天更换）的三手影响。然后用小鼠适应的甲型流感病毒（A/PR/8/34 H1N1）感染雄性成年后代，并在感染后 7 天评估肺部和骨髓免疫细胞的反应。母体第三只手接触含尼古丁的低功率（MLP）或高功率（MHP）电子蒸汽（分别为MLP+NIC和MHP+NIC）会增加肺部免疫细胞和骨髓中性粒细胞的百分比。有趣的是，与MSham流感对照组的后代相比，MLP+NIC组和MHP+NIC组的流感感染后代的肺泡巨噬细胞百分比较低，骨髓中性粒细胞的增加更为明显。流感感染还降低了肺部 CD4+ T 细胞的百分比，增加了肺部 CD8+ T 细胞的百分比，与母体暴露无关（MLP-/+NIC 和 MHP-/+NIC）。值得注意的是，MLP+NIC 和 MHP+NIC 都会导致肺 CD4+ T 细胞的活化减弱，但只有 MLP+NIC 会导致肺 CD8+ T 细胞的活化减弱。综上所述，我们首次发现，母体经第三只手接触电子蒸汽会对基线和流感感染时后代的肺部和骨髓免疫细胞反应产生显著而持久的影响。
Maternal thirdhand exposure to e-cigarette vapor alters lung and bone marrow immune cell responses in offspring in the absence or presence of influenza infection.
There is increasing evidence that thirdhand exposure to e-cigarette vapor (e-vapor) can have detrimental effects on the lungs. However, whether maternal exposure during pregnancy results in harmful changes to the offspring is unknown. Using two different e-cigarette settings (low vs. high power), BALB/c mice were subjected to thirdhand e-vapor (e-vapor deposited onto towels, towels changed daily) in the absence or presence of nicotine, before, during, and after pregnancy. Male adult offspring were then infected with mouse-adapted influenza A virus (A/PR/8/34 H1N1; Flu) and lung and bone marrow immune cell responses were assessed 7 days postinfection. Maternal thirdhand exposure to low-power (MLP) or high-power (MHP) e-vapor with nicotine (MLP + NIC and MHP + NIC, respectively) increased the percentage of lung immune cells and neutrophils in the bone marrow. Interestingly, Flu-infected offspring from MLP + NIC and MHP + NIC groups had lower percentages of lung alveolar macrophages and more pronounced increases in neutrophils in the bone marrow, when compared with offspring from MSham Flu controls. Flu infection also decreased the percentage of lung CD4+ T cells and increased the percentage of lung CD8+ T cells, irrespective of maternal exposure (MLP -/+ NIC and MHP -/+ NIC). Significantly, both MLP + NIC and MHP + NIC resulted in blunted activation of lung CD4+ T cells, but only MLP + NIC caused blunted activation of lung CD8+ T cells. Together, we show for the first time that maternal thirdhand exposure to e-vapor results in significant, long-lived effects on lung and bone marrow immune cell responses in offspring at baseline and response to Flu infection.NEW & NOTEWORTHY Maternal exposure to environmental residues of e-cigarette use has significant effects on immune cell responses in the lungs and bone marrow of offspring at both baseline and in response to influenza A virus (Flu) infection.
期刊介绍：
The American Journal of Physiology-Lung Cellular and Molecular Physiology publishes original research covering the broad scope of molecular, cellular, and integrative aspects of normal and abnormal function of cells and components of the respiratory system. Areas of interest include conducting airways, pulmonary circulation, lung endothelial and epithelial cells, the pleura, neuroendocrine and immunologic cells in the lung, neural cells involved in control of breathing, and cells of the diaphragm and thoracic muscles. The processes to be covered in the Journal include gas-exchange, metabolic control at the cellular level, intracellular signaling, gene expression, genomics, macromolecules and their turnover, cell-cell and cell-matrix interactions, cell motility, secretory mechanisms, membrane function, surfactant, matrix components, mucus and lining materials, lung defenses, macrophage function, transport of salt, water and protein, development and differentiation of the respiratory system, and response to the environment.
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