在哺乳动物细胞中构建同源糖蛋白的策略

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-09-25 DOI:10.1021/acs.bioconjchem.4c00361

Chang-Hee Lee, Hui Li, Ji Young Hyun, Injae Shin

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引用次数: 0

摘要

研究人员开发了一种将遗传密码扩增与生物正交连接技术相结合的通用策略，用于制备哺乳动物细胞表面的均一糖基化受体。利用这种方法，细胞表面催产素受体（OTR）与寡糖的共轭物在细胞中有效生成。细胞研究发现，与 OTR 相连的糖对于激动剂诱导的钙通量及其通过 OTR 介导的内吞作用内化到细胞中并不是必不可少的。

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Strategy for Construction of Homogeneous Glycoproteins in Mammalian Cells.

A general strategy that combines genetic code expansion with bio-orthogonal ligation techniques was developed and utilized to prepare homogeneously glycosylated receptors on the surface of mammalian cells. Using this approach, conjugates of the cell-surface oxytocin receptor (OTR) with oligosaccharides were efficiently generated in the cells. Cell studies revealed that glycans linked to the OTR are not essential for agonist-induced calcium flux and its internalization into cells via an OTR-mediated endocytosis.

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.