Hsa_circ_0087784 通过 miR-576-5p/CDCA4 轴增强小细胞肺癌的进展。

IF 2.3 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

American Journal of the Medical Sciences Pub Date : 2025-03-01 DOI:10.1016/j.amjms.2024.09.002

Bin Shang MD, Long Li MD, Gang Wang MD, Gang Liu MD, Xiaosong Yang MD, Jian Gao MD, Weiwei Yin MD

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引用次数: 0

摘要

背景：环状 RNA（circRNA）属于共价封闭的单链 RNA 家族，与癌症进展有关联。以前的研究曾报道 hsa_circ_0087784 在乳腺癌中异常表达。方法：我们使用 RT-qPCR 和 FISH 检测了 hsa_circ_0087784 在 NSCLC 细胞和组织样本中的表达。方法：我们采用 RT-qPCR 和 FISH 方法检测了 hsa_circ_0087784 在 NSCLC 细胞和组织样本中的表达，并使用双荧光素酶报告实验确定了 hsa_circ_0087784 的下游靶标。Transwell迁移、5-乙炔基-2´-脱氧尿苷和CCK-8试验用于检测迁移和增殖。肿瘤发生和转移试验用于确定 hsa_circ_0087784 在小鼠体内肿瘤异种移植模型中对 NSCLC 进展的作用：结果：我们发现，hsa_circ_0087784在NSCLC组织样本和细胞系中的表达水平很高。下调 hsa_circ_0087784 可抑制 NSCLC 细胞的增殖和迁移。我们的双荧光素酶报告实验发现，miR-576-5p 和 CDCA4 是 hsa_circ_0087784 的下游靶标。CDCA4 的过表达或 miR-576-5p 的抑制逆转了 hsa_circ_0087784 沉默对 NSCLC 细胞迁移和 EMT 相关蛋白表达水平的影响：我们的研究结果表明，下调hsa_circ_0087784可通过调控CDCA4的表达和miR-576-5psonging抑制NSCLC的转移和进展。

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Hsa_circ_0087784 enhances non-small cell lung cancer progression via the miR-576-5p/CDCA4 axis

Background

Circular RNAs (circRNAs) belong to a family of covalently closed single-stranded RNAs that have been implicated in cancer progression. Previous studies have reported that hsa_circ_0087784 was abnormally expressed in breast cancer. However, the role of hsa_circ_0087784 in non-small cell lung cancer (NSCLC) is unknown.

Methods

Here, we used RT-qPCR and FISH to examine hsa_circ_0087784 expression in NSCLC cells and tissue samples. The dual-luciferase reporter assay was used to identify downstream targets of hsa_circ_0087784. Transwell migration, 5-ethynyl-2´-deoxyuridine, and CCK-8 assays were used to examine migration and proliferation. Tumorigenesis and metastasis assays were used to determine the role of hsa_circ_0087784 in NSCLC progression in a mouse tumor xenograft model in vivo.

Results

We found that hsa_circ_0087784 was expressed at significantly high levels in NSCLC tissue samples and cell lines. Downregulation of hsa_circ_0087784 suppressed NSCLC cellular proliferation, as well as migration. Our dual-luciferase reporter assay revealed that miR-576-5p and CDCA4 were downstream targets of hsa_circ_0087784. CDCA4 overexpression or miR-576-5p suppression reversed the effects of hsa_circ_0087784 silencing on NSCLC cell migration, and EMT-related protein expression levels.

Conclusion

Our findings suggested that downregulation of hsa_circ_0087784 inhibited NSCLC metastasis and progression through the regulation of CDCA4 expression and miR-576-5p sponging.

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来源期刊

American Journal of the Medical Sciences 医学-医学：内科

CiteScore

4.40

自引率

0.00%

发文量

303

审稿时长

1.5 months

期刊介绍： The American Journal of The Medical Sciences (AJMS), founded in 1820, is the 2nd oldest medical journal in the United States. The AJMS is the official journal of the Southern Society for Clinical Investigation (SSCI). The SSCI is dedicated to the advancement of medical research and the exchange of knowledge, information and ideas. Its members are committed to mentoring future generations of medical investigators and promoting careers in academic medicine. The AJMS publishes, on a monthly basis, peer-reviewed articles in the field of internal medicine and its subspecialties, which include: Original clinical and basic science investigations Review articles Online Images in the Medical Sciences Special Features Include: Patient-Centered Focused Reviews History of Medicine The Science of Medical Education.