鱼鳞中的皮质醇在长期储存期间保持稳定。

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Conservation Physiology Pub Date : 2024-09-19 eCollection Date: 2024-01-01 DOI:10.1093/conphys/coae065
Christina O'Toole, Philip White, Conor T Graham, Caitlin Conroy, Deirdre Brophy
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引用次数: 0

摘要

测量鱼类鳞片中的皮质醇作为野生种群中慢性压力的非侵入性指标引起了广泛关注。对于许多具有管理和保护意义的鱼类物种来说,通过将皮质醇测量结果与生活史信息相结合,现有的大量鳞片收集可提供个体应激反应的扩展记录。然而,目前尚不清楚皮质醇在鳞片储存期间的保存情况。为了研究鳞片皮质醇的稳定性,我们将个体养殖大西洋鲑(Salmo salar)的鳞片在 50°C 的烘箱中储存 2 到 12 周,从而加速了潜在的降解。我们发现,鳞片皮质醇浓度与储存时间或储存温度均无明显关系。来自同一条鱼的鳞片中的皮质醇浓度是一致的(18.54-21.82 纳克/克-1;变异系数 (CV) = 3.6%),这表明即使鳞片储存时间不同或储存条件不同,也能可靠地量化鳞片中的皮质醇。我们还使用大西洋鲑鱼鳞片在纸质信封中室温下储存 3 到 32 年,对实时储存的影响进行了研究,结果发现鳞片皮质醇浓度与储存时间之间没有显著关系。鳞片皮质醇浓度范围为 4.05 至 135.37 纳克/克-1,个体间变异水平较高(CV = 61%)。鉴于鳞片皮质醇在长期储存过程中不会降解,历史鳞片采集和描述鱼类生活史的相关数据有可能被用于开发鱼类种群生理反应的生物指标。要了解鳞片皮质醇的变异性及其生物学意义,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cortisol in fish scales remains stable during extended periods of storage.

Measurement of cortisol in fish scales is attracting considerable attention as a non-invasive indicator of chronic stress in wild populations. For many fish species of management and conservation interest, extensive scale collections exist that could provide extended records of individual stress responses, by combining cortisol measurements with life history information. However, it is not yet known how well cortisol is preserved in the scale during storage. To investigate the stability of scale cortisol, we accelerated potential degradation by storing scales from an individual farmed Atlantic salmon (Salmo salar) in an oven at 50°C for between 2 and 12 weeks. We found no significant relationship between scale cortisol concentration and either storage time or storage temperature. Cortisol concentrations in scales from the same fish were consistent (18.54-21.82 ng. g-1; coefficient of variation (CV) = 3.6%), indicating that scale cortisol can be reliably quantified, even in scales stored for varying periods of time or under different conditions. We also examined the effects of storage in real time using Atlantic salmon scales that were stored in paper envelopes at room temperature for between 3 and 32 years and found no significant relationship between scale cortisol concentration and storage time. Scale cortisol concentrations ranged from 4.05 to 135.37 ng.g-1 and levels of between-individual variability were high (CV = 61%). Given that scale cortisol does not degrade during long-term storage, historical scale collections and associated data describing fish life histories could potentially be used to develop bioindicators of physiological responses in fish populations. Further research is needed to understand scale cortisol variability and its biological relevance.

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来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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