成纤维细胞干扰素和重组γ干扰素对酶酶酶诱导的小鼠腹腔巨噬细胞硝基蓝四唑还原的影响。

E von der Lippe, S Frøland, H Rollag, M Degre
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引用次数: 2

摘要

用成纤维细胞干扰素(IFN)制剂或重组γ -IFN (rifn - α)培养小鼠腹膜巨噬细胞(MPM) 1,24或48小时。然后测量酶生酶诱导的这些MPM中硝基蓝四氮唑(NBT)的还原。当培养时间为1 h时,成纤维细胞IFN增强了MPM的NBT还原能力。当培养时间延长至48 h时,记录了成纤维细胞IFN处理MPM抑制NBT还原的情况。在NBT减少方面,rifn - α对MPM的影响较小。只有当中等剂量的rifn - α (10 U/ml)处理MPM 48小时时,才记录到NBT减少增强。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of fibroblast interferon and recombinant gamma interferon on zymosan-induced nitroblue tetrazolium reduction in mouse peritoneal macrophages.

Mouse peritoneal macrophages (MPM) were cultivated with a fibroblast interferon (IFN) preparation or recombinant gamma-IFN (rIFN-alpha) for 1, 24 or 48 h. The zymosan-induced reduction of nitroblue tetrazolium (NBT) in these MPM was then measured. Fibroblast IFN enhanced the NBT reducing capacity of MPM when the incubation period was 1 h. When the incubation period was extended to 48 h, a suppressed NBT reduction by fibroblast IFN treated MPM was recorded. The influence of rIFN-alpha on MPM with regard to NBT reduction was minor. Only when the MPM were treated with a moderate dose of rIFN-alpha (10 U/ml) for 48 h was an enhanced NBT reduction recorded.

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