生长中的非人灵长类前卵泡基因表达的动态变化。

IF 2.5 4区 生物学 Q3 CELL BIOLOGY
Physiological genomics Pub Date : 2024-11-01 Epub Date: 2024-09-23 DOI:10.1152/physiolgenomics.00023.2024
Catherine A VandeVoort, Charles L Chaffin, Peter Z Schall, Keith E Latham
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引用次数: 0

摘要

卵巢前位卵泡的生长是一个复杂的过程,很难研究,尤其是在人类和非人灵长类动物中。了解窦前卵泡的发育阶段是采用新方法调节生殖的关键。本研究通过对卵母细胞、积壳细胞和颗粒细胞进行 RNA 测序,分析了从成年猕猴雌性体内获得的三种大小的发育中的窦前卵泡。这项研究的总体目标是确定在没有外源激素刺激的情况下,非人灵长类动物前额期卵泡逐渐增大时,卵母细胞、颗粒细胞和积层细胞中基因表达的关键发育变化。在此期间,三种细胞类型中只有相对较少的基因的 mRNA 表达水平发生了变化。大多数已确定的差异表达基因(DEGs)在颗粒细胞中减少,或在积聚细胞中增加。虽然观察到的 DEGs 数量较少,但这些 DEGs 表明了对积液细胞和颗粒细胞中不同上游调节因子的预测影响。这项研究尤为重要,因为它首次在医学相关模型中显示了前卵泡生长过程中基因表达的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic changes in gene expression of growing nonhuman primate antral follicles.

The growth of the ovarian antral follicle is a complex process that is difficult to study, especially in human and nonhuman primates. Understanding the antral stage of development is key to new approaches to regulating reproduction. This study analyzed cohorts of three sizes of developing antral follicles obtained from adult rhesus macaque females using RNA sequencing of oocytes and cumulus and granulosa cells. The overall objective of this study was to identify key developmental changes in gene expression in oocytes, granulosa, and cumulus cells, as nonhuman primate antral stage follicles transition through progressively larger sizes in the absence of exogenous hormonal stimulation. Only a relatively small number of genes displayed altered mRNA expression levels in any of the three cell types during this period. Most of the identified differentially expressed genes (DEGs) decreased in the granulosa cells or increased in the cumulus cells. Although the number of DEGs observed was small, these DEGs indicate predicted effects on distinct upstream regulators in the cumulus and granulosa cells. This study is particularly important because it shows for the first time the gene expression changes during antral follicle growth in a medically relevant model.NEW & NOTEWORTHY Changes in gene expression in oocytes, granulosa, and cumulus cells were determined in nonhuman primate antral stage ovarian follicles transitioning through progressively larger sizes without exogenous hormonal stimulation. Only a small number of genes displayed altered mRNA expression levels in any of the three cell types. Most of the differentially expressed genes (DEGs) decreased in granulosa cells or increased in cumulus cells. These results identified upstream regulators of antral follicle development.

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来源期刊
Physiological genomics
Physiological genomics 生物-生理学
CiteScore
6.10
自引率
0.00%
发文量
46
审稿时长
4-8 weeks
期刊介绍: The Physiological Genomics publishes original papers, reviews and rapid reports in a wide area of research focused on uncovering the links between genes and physiology at all levels of biological organization. Articles on topics ranging from single genes to the whole genome and their links to the physiology of humans, any model organism, organ, tissue or cell are welcome. Areas of interest include complex polygenic traits preferably of importance to human health and gene-function relationships of disease processes. Specifically, the Journal has dedicated Sections focused on genome-wide association studies (GWAS) to function, cardiovascular, renal, metabolic and neurological systems, exercise physiology, pharmacogenomics, clinical, translational and genomics for precision medicine, comparative and statistical genomics and databases. For further details on research themes covered within these Sections, please refer to the descriptions given under each Section.
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