非血红素铁(II)依赖性氧化酶/脱羧酶的异腈生物合成。

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-06-29 DOI:10.1016/bs.mie.2024.06.002
Antonio Del Rio Flores, Rui Zhai, Wenjun Zhang
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引用次数: 0

摘要

在合成化学和化学生物学中,异腈基团是一种结构紧凑、富含电子的分子基团,它是一种常见的构筑基块,具有与生物相同的功能。从不同的生物体中分离出了数百种含有异腈基团的天然产物,它们具有令人感兴趣的生物活性特性。我们最近在一些放线菌中发现了一个保守的生物合成基因簇,这突出了一种新的异腈形成酶途径,其中涉及非血红素铁(II)和α-酮戊二酸依赖性二氧酶。在本章中,我们将重点介绍在了解和探究非血红素铁(II)和α-酮戊二酸二氧酶合成异腈的生物合成机制方面的最新进展。我们将首先介绍如何通过异源表达、纯化、合成策略和体外生化/动力学表征来利用异腈酶机制。然后,我们将介绍一种可推广的策略,通过结合各种光谱方法来探究异腈的形成机制。本章还将介绍通过使用生物合成途径酶实施耦合测定来研究其他酶同源物的策略。在本章的最后,我们将讨论在理解异腈合成和异腈合成工程方面当前面临的挑战和未来的发展方向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isonitrile biosynthesis by non-heme iron(II)-dependent oxidases/decarboxylases.

The isonitrile group is a compact, electron-rich moiety coveted for its commonplace as a building block and bioorthogonal functionality in synthetic chemistry and chemical biology. Hundreds of natural products containing an isonitrile group with intriguing bioactive properties have been isolated from diverse organisms. Our recent discovery of a conserved biosynthetic gene cluster in some Actinobacteria species highlighted a novel enzymatic pathway to isonitrile formation involving a non-heme iron(II) and α-ketoglutarate-dependent dioxygenase. Here, we focus this chapter on recent advances in understanding and probing the biosynthetic machinery for isonitrile synthesis by non-heme iron(II) and α-ketoglutarate-dependent dioxygenases. We will begin by describing how to harness isonitrile enzymatic machinery through heterologous expression, purification, synthetic strategies, and in vitro biochemical/kinetic characterization. We will then describe a generalizable strategy to probe the mechanism for isonitrile formation by combining various spectroscopic methods. The chapter will also cover strategies to study other enzyme homologs by implementing coupled assays using biosynthetic pathway enzymes. We will conclude this chapter by addressing current challenges and future directions in understanding and engineering isonitrile synthesis.

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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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