评估三种人为活动对科特迪瓦阿比让市冈比亚按蚊物种组成和除虫菊酯抗药性机制的影响。

IF 1.6 3区 农林科学 Q2 ENTOMOLOGY
Alain K Y Kacou, Constant Edi, Behi K Fodjo, Firmain N Yokoly, Julien B Z Zahouli, Ives J K Kouamé, David Malone, Benjamin G Koudou
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引用次数: 0

摘要

在阿比让的三个城区(蔬菜种植区、工业区和居民区)评估了冈比亚按蚊(双翅目:疟原虫)对拟除虫菊酯的抗药性和抗药性机制。在预先接触或未预先接触胡椒基丁醚(PBO)的情况下,评估了对拟除虫菊酯(溴氰菊酯、氯菊酯和阿尔法氯菊酯)的敏感性。使用短穿插核元素聚合酶链式反应(SINE PCR)鉴定了冈比亚按蚊复合体成员,并通过实时聚合酶链式反应(PCR)确定了抗药性机制(kdr-west、kdr-east 和 ace-1)和代谢基因表达(CYP6P5、CYP6M2、CYP6P3、CYP6P4)。在布埃港(蔬菜种植区)和 Treichville(工业区)观察到对溴氰菊酯、氯菊酯和甲氰菊酯的高抗性,而在阿波波(住宅区)发现对溴氰菊酯的中等抗性和对甲氰菊酯和氯菊酯的高抗性。在所有地点,预先接触 PBO 和拟除虫菊酯都会增加死亡率。在 Treichville,预先接触 PBO 可恢复对溴氰菊酯的敏感性,但在 Port-Bouet 和 Abobo 则不然。在特雷奇维尔,严格意义上的冈比亚蚁 Giles(s.s.)占主导地位(92.9%),而在阿波波和布埃港,冈比亚蚁 coluzzii Giles 占主导地位(分别为 73.6% 和 66.4%)。kdr-west变异频率最高的是阿波波的冈比亚鳗(42.8%),其次是布埃港的冈比亚鳗(40%)和特雷什维尔的冈比亚鳗(35.6%)。在 Coluzzii 中,kdr-West 变异频率最高的是布埃港(48.2%),其次是阿波波(28.00%)和特雷什维尔(21.4%)。在特雷奇维尔(4.1%)和布埃港(2.1%)的冈比亚鳗中,kdr-东部的突变频率较低,而在阿波波的冈比亚鳗中则没有突变频率。与基苏木相比,工业区和蔬菜种植区的 CYP6P3 和 CYP6M2 表达过高。这项研究表明,冈比亚虫的分布和对除虫菊酯的抗性受到人类活动的影响。特雷奇维尔的工业区有利于冈比亚疟原虫的繁殖,而阿波波的居民区和布埃港的蔬菜种植区则以科鲁兹疟原虫为主。特雷奇维尔和布埃港的抗药性与 kdr(西部和东部)基因和代谢基因有关,而在居民区,只观察到 kdr 西部基因。这些数据表明,PBO+溴氰菊酯浸渍蚊帐可以帮助控制疟疾,使科特迪瓦和其他非洲城市的工业区受益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessing the effects of three anthropogenic activities on the mosquito, Anopheles gambiae s.l. species composition and pyrethroid resistance mechanisms in Abidjan city, Côte d'Ivoire.

Pyrethroid resistance and mechanisms for resistance for Anopheles gambiae sensus lato (s.l.) (Diptera: Culicidae) Giles, were assessed in three urban areas (vegetable farming, industrial and residential) of Abidjan. Susceptibility to pyrethroids (deltamethrin, permethrin and alphacypermethrin), with and without piperonyl butoxide (PBO) pre-exposure was evaluated. Anopheles gambiae complex members were identified using Short Interspersed Nuclear Elements PCR (SINE PCR), and resistance mechanisms (kdr-west, kdr-east and ace-1) and metabolic gene expression (CYP6P5, CYP6M2, CYP6P3, CYP6P4) were determined by real-time polymerase chain reaction (PCR). High resistance to deltamethrin, permethrin and alphacypermethrin was observed in Port-Bouet (vegetable farming) and Treichville (industrial site), whereas moderate resistance to deltamethrin and high resistance to alphacypermethrin and permethrin were found in Abobo (residential site). Pre-exposure to PBO with pyrethroid increased mortalities in all sites. In Treichville, pre-exposure to PBO restored susceptibility to deltamethrin, but not in Port-Bouet or Abobo. In Treichville, An. gambiae Giles sensu stricto (s.s.) was predominant (92.9%), whereas in Abobo and Port-Bouet, An. coluzzii Giles was predominant (73.6% and 66.4%, respectively). The highest kdr-west mutation frequency was in An. gambiae s.s. (42.8%) from Abobo, followed by An. gambiae s.s. (40%) from Port-Bouet and An. gambiae s.s. (35.6%) from Treichville. In An. coluzzii, the highest kdr-west mutation frequency was in Port-Bouet (48.2%), followed by Abobo (28.00%) and Treichville (21.4%). Mutation frequencies of kdr-east were lower in An. gambiae s.s. from Treichville (4.1%) and Port-Bouet (2.1%) and absent in An. gambiae from Abobo. In industrial and vegetable farming areas, CYP6P3 and CYP6M2 were overexpressed compared with Kisumu. The study suggests An. gambiae s.l. distribution and pyrethroid resistance are influenced by human activities. Treichville's industrial area favoured An. gambiae s.s., whereas Abobo's residential and Port-Bouet's vegetable farming areas were dominated by An. coluzzii. Resistance in Treichville and Port-Bouet was associated with kdr (west and east) genes and metabolic genes, whereas in residential areas, only kdr-west genes were observed. These data suggest that PBO + deltamethrin impregnated nets could aid malaria control, benefiting industrial areas of Côte d'Ivoire and other African cities.

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来源期刊
Medical and Veterinary Entomology
Medical and Veterinary Entomology 农林科学-昆虫学
CiteScore
3.70
自引率
5.30%
发文量
65
审稿时长
12-24 weeks
期刊介绍: Medical and Veterinary Entomology is the leading periodical in its field. The Journal covers the biology and control of insects, ticks, mites and other arthropods of medical and veterinary importance. The main strengths of the Journal lie in the fields of: -epidemiology and transmission of vector-borne pathogens changes in vector distribution that have impact on the pathogen transmission- arthropod behaviour and ecology- novel, field evaluated, approaches to biological and chemical control methods- host arthropod interactions. Please note that we do not consider submissions in forensic entomology.
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