Shengwei Zhuang, Zhekun Huang, Hongkai Fan, Zhirong Wu, Han Liu
{"title":"LINC01232 通过 p300/H3K27ac 促进 ARNTL2 的转录激活并抑制 CRC 细胞的铁变态反应。","authors":"Shengwei Zhuang, Zhekun Huang, Hongkai Fan, Zhirong Wu, Han Liu","doi":"10.1080/17501911.2024.2387528","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> This study investigated the role of lncRNA <i>LINC01232</i> in ferroptosis of colorectal cancer (CRC).<b>Materials & methods:</b> Real time quantitative polymerase chain reaction or western blot experiments were performed to examine relevant mRNAs and proteins expression. The kit assays evaluated malondialdehyde, iron, Fe<sup>2+</sup> and glutathione levels. ROS levels were verified by flow cytometry. Chromatin immunoprecipitation and RNA immunoprecipitation analysis monitored the correlation among <i>LINC01232</i>, H3K27ac, p300 and ARNTL2.<b>Results:</b> <i>LINC01232</i> or <i>ARNTL2</i> knockdown facilitated erastin-induced ferroptosis. The interaction between <i>LINC01232</i> and p300 resulted in the enhancement of H3K27ac levels at <i>ARNTL2</i> promoter to promote <i>ARNTL2</i> transcriptional activity. <i>ARNTL2</i> overexpression reversed the promoting effect of <i>LINC01232</i> knockdown on ferroptosis.<b>Conclusion:</b> <i>LINC01232</i> inhibited the ferroptosis in CRC by epigenetically upregulating the transcriptional activity of <i>ARNTL2</i>.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":"16 15-16","pages":"1097-1115"},"PeriodicalIF":3.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418281/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>LINC01232</i> promotes <i>ARNTL2</i> transcriptional activation and inhibits ferroptosis of CRC cells through p300/H3K27ac.\",\"authors\":\"Shengwei Zhuang, Zhekun Huang, Hongkai Fan, Zhirong Wu, Han Liu\",\"doi\":\"10.1080/17501911.2024.2387528\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> This study investigated the role of lncRNA <i>LINC01232</i> in ferroptosis of colorectal cancer (CRC).<b>Materials & methods:</b> Real time quantitative polymerase chain reaction or western blot experiments were performed to examine relevant mRNAs and proteins expression. The kit assays evaluated malondialdehyde, iron, Fe<sup>2+</sup> and glutathione levels. ROS levels were verified by flow cytometry. Chromatin immunoprecipitation and RNA immunoprecipitation analysis monitored the correlation among <i>LINC01232</i>, H3K27ac, p300 and ARNTL2.<b>Results:</b> <i>LINC01232</i> or <i>ARNTL2</i> knockdown facilitated erastin-induced ferroptosis. The interaction between <i>LINC01232</i> and p300 resulted in the enhancement of H3K27ac levels at <i>ARNTL2</i> promoter to promote <i>ARNTL2</i> transcriptional activity. <i>ARNTL2</i> overexpression reversed the promoting effect of <i>LINC01232</i> knockdown on ferroptosis.<b>Conclusion:</b> <i>LINC01232</i> inhibited the ferroptosis in CRC by epigenetically upregulating the transcriptional activity of <i>ARNTL2</i>.</p>\",\"PeriodicalId\":11959,\"journal\":{\"name\":\"Epigenomics\",\"volume\":\"16 15-16\",\"pages\":\"1097-1115\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11418281/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/17501911.2024.2387528\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/13 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17501911.2024.2387528","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/13 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
LINC01232 promotes ARNTL2 transcriptional activation and inhibits ferroptosis of CRC cells through p300/H3K27ac.
Aim: This study investigated the role of lncRNA LINC01232 in ferroptosis of colorectal cancer (CRC).Materials & methods: Real time quantitative polymerase chain reaction or western blot experiments were performed to examine relevant mRNAs and proteins expression. The kit assays evaluated malondialdehyde, iron, Fe2+ and glutathione levels. ROS levels were verified by flow cytometry. Chromatin immunoprecipitation and RNA immunoprecipitation analysis monitored the correlation among LINC01232, H3K27ac, p300 and ARNTL2.Results:LINC01232 or ARNTL2 knockdown facilitated erastin-induced ferroptosis. The interaction between LINC01232 and p300 resulted in the enhancement of H3K27ac levels at ARNTL2 promoter to promote ARNTL2 transcriptional activity. ARNTL2 overexpression reversed the promoting effect of LINC01232 knockdown on ferroptosis.Conclusion:LINC01232 inhibited the ferroptosis in CRC by epigenetically upregulating the transcriptional activity of ARNTL2.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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