Paulo Victor Barbosa Eleutério Dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida Dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa
{"title":"早产对 LINE-1 启动子甲基化的影响","authors":"Paulo Victor Barbosa Eleutério Dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida Dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa","doi":"10.1080/17501911.2024.2397329","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.<b>Materials & methods:</b> Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.<b>Results:</b> Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.<b>Conclusion:</b> This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"1253-1264"},"PeriodicalIF":3.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486321/pdf/","citationCount":"0","resultStr":"{\"title\":\"Impact of prematurity on LINE-1 promoter methylation.\",\"authors\":\"Paulo Victor Barbosa Eleutério Dos Santos, Aline de Araújo Brasil, Leo Travassos Vieira Milone, Georgia Chalfun, Stephanie Cristina Alves de Oliveira Saide, Margarida Dos Santos Salú, Mariana Barros Genuíno de Oliveira, Jaqueline Rodrigues Robaina, Fernanda Lima-Setta, Gustavo Rodrigues-Santos, Maria Clara de Magalhães-Barbosa, Antônio José Ledo Alves da Cunha, Arnaldo Prata-Barbosa\",\"doi\":\"10.1080/17501911.2024.2397329\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.<b>Materials & methods:</b> Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.<b>Results:</b> Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.<b>Conclusion:</b> This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.</p>\",\"PeriodicalId\":11959,\"journal\":{\"name\":\"Epigenomics\",\"volume\":\" \",\"pages\":\"1253-1264\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486321/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/17501911.2024.2397329\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/9/19 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/17501911.2024.2397329","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Impact of prematurity on LINE-1 promoter methylation.
Aim: Promoter methylation of LINE-1 may be affected by prematurity, but there is little evidence in the literature.Materials & methods: Blood from premature and full-term neonates on days 0, 5, 30 and 90 was analyzed for DNA methylation percentage in a promoter region of the LINE-1, after bisulfite conversion and pyrosequencing.Results: Premature infants, as a whole, showed significantly lower methylation percentage at birth, but this difference diminished over time. However, the subgroup of extremely premature (<28 weeks gestational age) had higher methylation percentages, similar to full-term newborns.Conclusion: This research underscores the critical role of prematurity on the methylation pattern of LINE-1. These findings underline the complexity of epigenetic regulation in prematurity and emphasize the need for further studies.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
