对快速生长的细长 Synechococcus 菌株进行代谢工程改造,以实现光营养生产烷烃。

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Vaibhav Srivastava, Aditya P Sarnaik, Pramod P Wangikar
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引用次数: 0

摘要

烷烃是高能碳氢化合物,被认为是下一代生物燃料。众所周知,蓝藻可天然合成 C15-C19 烷烃;然而,由于滴度太低,这一工艺在商业上并不可行。因此,为了利用这些光合平台改进烷烃的生产,我们在这里设计了三种新型的细长拟球藻分离物 PCC 11801、PCC 11802 和 IITB6。在 PCC 7942 的 PrbcL 启动子和 PCC 11801 的 PcpcB300、PpsbAI 和 PpsbAIII 等原生启动子的调控下,我们克隆了来自拉长藻 PCC 7942 的酰基-ACP 还原酶(ar)和醛脱甲酰氧合酶(ado)基因,从而构建了双基因 AAR-ADO 烷烃生物合成途径。这些基因被分别克隆在两个不同的启动子下,形成了一个工程菌株库。结果表明,新分离出的拉长藻工程菌株产生的烷烃量明显高于模式菌株 PCC 7942。在 BG-11 中,最高的烷烃产量为 4.1 mg/gDCW,而在 5X BG-11 中,最高的滴度为 31.5 mg/L,采用的是工程 IITB6 菌株(PcpcB300:ar:TrrnB::PrbcL:ado:TLac)。总之,该研究强调了新分离出的细长根杆菌菌株作为高效烷烃生产平台的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolic engineering of rapidly growing Synechococcus elongatus strains for phototrophic production of alkanes.

Alkanes are high-energy hydrocarbons that are foreseen as next generation biofuels. Cyanobacteria are known to naturally synthesize C15-C19 alkanes; however, the titers are too low to make this a commercially viable process. Therefore, to leverage these photosynthetic platforms for improved alkane production, here we engineered three novel isolates of Synechococcus elongatus PCC 11801, PCC 11802, and IITB6. The two gene AAR-ADO alkane biosynthesis pathway was constructed by cloning the genes for acyl-ACP reductase (aar) and aldehyde deformylating oxygenase (ado) from S. elongatus PCC 7942 under the regulation of PrbcL promoter from PCC 7942 and native promoters from PCC 11801 such as PcpcB300, PpsbAI, and PpsbAIII. The genes were separately cloned under two different promoters, creating a library of the engineered strains. The results indicated that the engineered strains of novel S. elongatus isolates produced significantly higher amounts of alkanes than the model strain PCC 7942. The highest alkane yield achieved was 4.1 mg/gDCW in BG-11, while the highest titer was 31.5 mg/L in 5X BG-11, with an engineered IITB6 strain (PcpcB300:aar:TrrnB::PrbcL:ado:TLac). Overall, the study highlights the potential of newly isolated S. elongatus strains as efficient alkane production platforms.

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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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