根据肽的质量分数确定氨基酸组成

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Kevin M. Downard, Robert B. Cody
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引用次数: 0

摘要

多肽的质量分数与其元素组成直接相关,因此也与氨基酸组成直接相关。这里证明,对于中小型多肽(5-7 个氨基酸)来说,单凭多肽的分数质量就能有效地识别或指示其组成,并能显著减少较大型多肽(8 个残基)的可行氨基酸组成的数量,从而包括或排除某些可能性。单独考虑多肽质量的整数部分有助于减少发现许多重复分数质量值的可能性。采用这种分数质量策略应有助于目前采用的肽鉴定方法,包括使用质量图数据搜索蛋白质组学应用的蛋白质数据库。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amino Acid Composition Determination From the Fractional Mass of Peptides

A peptide's fractional mass is directly associated with its elemental composition and thus amino acid composition. Here it is demonstrated that a peptide's fractional mass alone can be a useful identifier or indicator of that composition for small to mid-sized peptides (5–7 amino acids) and can significantly reduce the number of viable amino acid compositions for larger peptides (> 8 residues) to include or exclude certain possibilities. Separate consideration of the integer portion of the peptide's mass helps to reduce the number of possibilities where many duplicate fractional mass values are found. Adoption of this fractional mass strategy should aid approaches that are presently employed for peptide identification, including in the use of mass map data to search protein databases for proteomics applications.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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