用单蓝绿光控制有丝分裂细胞中染色体定位的光开关式 CENP-E 抑制剂

IF 4.1 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Kazuya Matsuo, Takashi Kikukawa, Tomonori Waku, Akio Kobori and Nobuyuki Tamaoki
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引用次数: 0

摘要

可逆光开关化学工具有助于开发生物医学领域的新方法。与紫外线相比,可见光的光毒性低、穿透深度深,因此是这种方法在生物领域应用的理想选择。在此,我们报告了一种可光开关的中心粒相关蛋白 E(CENP-E)抑制剂,这种抑制剂可在低能量蓝绿光(约 500 纳米)照射下进行控制。这种光开关工具使我们能够在亚细胞分辨率下控制 CENP-E 驱动的染色体移动和定位,而且光毒性很低。这项研究有助于开发一种独特的染色体工程技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A photoswitchable CENP-E inhibitor with single blue-green light to control chromosome positioning in mitotic cells†

A photoswitchable CENP-E inhibitor with single blue-green light to control chromosome positioning in mitotic cells†

A photoswitchable CENP-E inhibitor with single blue-green light to control chromosome positioning in mitotic cells†

Reversibly photoswitchable chemical tools have aided in the development of novel approaches in the biomedical field. The visible region of light should be ideal for the biological application of this approach because of its low phototoxicity and deep penetration depth compared to ultraviolet light. Herein, we report a photoswitchable centromere-associated protein E (CENP-E) inhibitor, which is controllable with low-energy blue-green light (around 500 nm) illumination. This photoswitchable tool enabled us to control CENP-E-driven chromosome movements and positioning at subcellular resolutions with low phototoxic effects. This study can contribute to the development of a unique technique for chromosome engineering.

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来源期刊
CiteScore
5.80
自引率
2.40%
发文量
129
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