Application of novel 3D culture device for human omental adipocytes and macrophages derived from surgery

Visceral adipose tissue plays a key role in the inflammation, inducing metabolic dysfunction. The culturing system of major components, adipocytes and adipose tissue macrophages (ATM) have been improved up to date, for example, the ceiling culture system, three-dimensional collagen gels and membrane mature adipocyte aggregate cultures (MAAC). Here we applied for a novel 3D culture device, cellbed of human omental adipocytes with ATM derived from surgery and presented the first morphological report. The pilot study has the limitation of resolution due to the thickness, however, the simple method would be a convenient assay to detect their morphological alteration after surgery. In addition, the combination of cell morphological observation on the cellbed and adipocytokine secretion capability would give an insight into the mechanism of adipose tissue-derived inflammation after surgery. The cellbed composed of high-grade silica glass fiver has been already provided the morphological study with the critical signaling transduction in human oral cancer research. The novel simple 3D device will prevail to spread the practical diversity in the functional clinical research.