寻找 Sli Locus

IF 2.3 3区 农林科学 Q1 AGRONOMY
Ernst-Jan Eggers, Ying Su, Sjaak A. W. van Heusden, Michiel E. de Vries, Christian W. B. Bachem, Richard G. F. Visser, Pim Lindhout
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引用次数: 0

摘要

马铃薯育种的遗传增益受到栽培马铃薯杂合四倍体基因组的限制。最近在二倍体马铃薯育种方面所做的努力有望提高遗传增益,使遗传和引种育种更加直接。二倍体F1杂交马铃薯育种依赖于通过重复自交创造二倍体近交系的能力。然而,二倍体马铃薯的自交受到由S-locus编码的配子体自相容性系统的阻碍,该系统阻止自花粉受精。尽管如此,自交不亲和的二倍体马铃薯基因型仍然存在,并被用于培育近交系。S-locus抑制剂(Sli)基因是一种显性基因,在二倍体马铃薯中具有很强的自相容性,该基因以前被绘制在第12号染色体上。虽然 Sli 基因已经被确定并表征出来,但最繁琐的挑战是在克隆该基因之前开发出最佳的表型方法和遗传群体。为此,我们制定了一套有效的表型鉴定方案,以确定合适的亲本,并建立 Sli 基因的二倍体分离群体。我们的研究表明,准确的表型方法对于区分育性干扰因素和自相容性至关重要。此外,我们还发现 Sli 基因座在 12 号染色体上表现出极端的分离畸变。最后,我们利用这些研究成果培育出了三个Sli基因分离的F1群体,随后我们利用这些群体鉴定了Sli基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The Quest for the Sli Locus

The Quest for the Sli Locus

Genetic gain in potato breeding is limited by the heterozygous tetraploid genome of cultivated potato. Recent efforts to breed potato at the diploid level promise to improve genetic gain and allow more straightforward genetics and introgression breeding. Diploid F1 hybrid potato breeding relies on the ability to create diploid inbred lines via repeated self-fertilization. However, self-fertilization of diploid potato is hampered by a gametophytic self-incompatibility system encoded by the S-locus that prevents fertilization by self-pollen. Nonetheless, self-compatible diploid potato genotypes exist and have been used to create inbred lines. The S-locus inhibitor (Sli) gene is a dominant gene that provides strong self-compatibility in diploid potato and was previously mapped to Chromosome 12. While the Sli gene has already been identified and characterized, the most tedious challenge was to develop the optimal phenotyping methods and genetic populations preceding the cloning of this gene. To this end, we developed an effective phenotyping protocol to identify suitable parents and create diploid populations segregating for Sli. We show that an accurate phenotyping method is crucial to discriminate between confounding fertility factors and self-compatibility. In addition, we found that the Sli locus shows extreme segregation distortion on Chromosome 12. Finally, we used these insights to develop three F1 populations that segregate for Sli, which we later used for the identification of the Sli gene.

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来源期刊
Potato Research
Potato Research AGRONOMY-
CiteScore
5.50
自引率
6.90%
发文量
66
审稿时长
>12 weeks
期刊介绍: Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as: Molecular sciences; Breeding; Physiology; Pathology; Nematology; Virology; Agronomy; Engineering and Utilization.
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