Tiago Sartor, Natasha Ruschel Soares, Vítor da Silveira Falavigna, Luis Fernando Revers, Eliane R. Santarém, Leandro V. Astarita
{"title":"细菌诱导剂 XTH 和水杨酸调控马铃薯防御相关基因的表达","authors":"Tiago Sartor, Natasha Ruschel Soares, Vítor da Silveira Falavigna, Luis Fernando Revers, Eliane R. Santarém, Leandro V. Astarita","doi":"10.1007/s11540-024-09796-z","DOIUrl":null,"url":null,"abstract":"<p>Potato (<i>Solanum tuberosum</i>) is a major staple food crop susceptible to numerous pathogens and pests. Utilising biotic elicitors offers a promising integrative approach for crop management, potentially reducing losses and chemical treatments. One such elicitor, an inactive suspension of <i>Xanthomonas axonopodis</i> (XTH), has previously been shown to enhance potato resistance against pathogens, like the bacterium causing blackleg disease. However, the underlying mechanism of this resistance remains unclear. Thus, this study investigated the effect of XTH on the defence metabolism in potato plants and compared it to the response elicited by exogenous salicylic acid (SA), a well-known defence-signalling molecule. We analysed the expression of marker genes for defence response pathways, including JA/ET-responsive genes (<i>StPin2</i>, <i>StERF1</i>, and <i>StJAZ1/TIFY10A-like</i>) and SA-responsive genes (<i>StPR-1b</i>, <i>StPR-2</i>, and <i>StChtA</i>). Potato plants were treated with either SA and XTH, and both treated and systemic leaves were analysed. XTH upregulated all analysed genes locally and systemically within the first 24 h, except for <i>StChtA</i>. The XTH-mediated upregulation of <i>StPAL</i> and <i>Pin2</i> genes suggests this elicitor might trigger responses via the jasmonic acid pathway. Exogenous application of SA induced the systemic expression of <i>StPR</i>, <i>StChtA</i>, <i>StJAZ1/TIFY10A-like</i>, and <i>StERF</i> in potato plants. Our results indicate that XTH modulates the expression of defence-related genes in potato plants by simultaneously activating both the salicylic acid and jasmonic acid signalling pathways. This dual activation suggests that XTH could be a valuable resource for crop management in potato cultivation, potentially reducing the need for chemical pesticides.</p>","PeriodicalId":20378,"journal":{"name":"Potato Research","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacterial Elicitor XTH and Salicylic Acid Regulate the Expression of Defence-Related Genes in Potato\",\"authors\":\"Tiago Sartor, Natasha Ruschel Soares, Vítor da Silveira Falavigna, Luis Fernando Revers, Eliane R. Santarém, Leandro V. Astarita\",\"doi\":\"10.1007/s11540-024-09796-z\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Potato (<i>Solanum tuberosum</i>) is a major staple food crop susceptible to numerous pathogens and pests. Utilising biotic elicitors offers a promising integrative approach for crop management, potentially reducing losses and chemical treatments. One such elicitor, an inactive suspension of <i>Xanthomonas axonopodis</i> (XTH), has previously been shown to enhance potato resistance against pathogens, like the bacterium causing blackleg disease. However, the underlying mechanism of this resistance remains unclear. Thus, this study investigated the effect of XTH on the defence metabolism in potato plants and compared it to the response elicited by exogenous salicylic acid (SA), a well-known defence-signalling molecule. We analysed the expression of marker genes for defence response pathways, including JA/ET-responsive genes (<i>StPin2</i>, <i>StERF1</i>, and <i>StJAZ1/TIFY10A-like</i>) and SA-responsive genes (<i>StPR-1b</i>, <i>StPR-2</i>, and <i>StChtA</i>). Potato plants were treated with either SA and XTH, and both treated and systemic leaves were analysed. XTH upregulated all analysed genes locally and systemically within the first 24 h, except for <i>StChtA</i>. The XTH-mediated upregulation of <i>StPAL</i> and <i>Pin2</i> genes suggests this elicitor might trigger responses via the jasmonic acid pathway. Exogenous application of SA induced the systemic expression of <i>StPR</i>, <i>StChtA</i>, <i>StJAZ1/TIFY10A-like</i>, and <i>StERF</i> in potato plants. Our results indicate that XTH modulates the expression of defence-related genes in potato plants by simultaneously activating both the salicylic acid and jasmonic acid signalling pathways. This dual activation suggests that XTH could be a valuable resource for crop management in potato cultivation, potentially reducing the need for chemical pesticides.</p>\",\"PeriodicalId\":20378,\"journal\":{\"name\":\"Potato Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Potato Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11540-024-09796-z\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Potato Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11540-024-09796-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Bacterial Elicitor XTH and Salicylic Acid Regulate the Expression of Defence-Related Genes in Potato
Potato (Solanum tuberosum) is a major staple food crop susceptible to numerous pathogens and pests. Utilising biotic elicitors offers a promising integrative approach for crop management, potentially reducing losses and chemical treatments. One such elicitor, an inactive suspension of Xanthomonas axonopodis (XTH), has previously been shown to enhance potato resistance against pathogens, like the bacterium causing blackleg disease. However, the underlying mechanism of this resistance remains unclear. Thus, this study investigated the effect of XTH on the defence metabolism in potato plants and compared it to the response elicited by exogenous salicylic acid (SA), a well-known defence-signalling molecule. We analysed the expression of marker genes for defence response pathways, including JA/ET-responsive genes (StPin2, StERF1, and StJAZ1/TIFY10A-like) and SA-responsive genes (StPR-1b, StPR-2, and StChtA). Potato plants were treated with either SA and XTH, and both treated and systemic leaves were analysed. XTH upregulated all analysed genes locally and systemically within the first 24 h, except for StChtA. The XTH-mediated upregulation of StPAL and Pin2 genes suggests this elicitor might trigger responses via the jasmonic acid pathway. Exogenous application of SA induced the systemic expression of StPR, StChtA, StJAZ1/TIFY10A-like, and StERF in potato plants. Our results indicate that XTH modulates the expression of defence-related genes in potato plants by simultaneously activating both the salicylic acid and jasmonic acid signalling pathways. This dual activation suggests that XTH could be a valuable resource for crop management in potato cultivation, potentially reducing the need for chemical pesticides.
期刊介绍:
Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as:
Molecular sciences;
Breeding;
Physiology;
Pathology;
Nematology;
Virology;
Agronomy;
Engineering and Utilization.