细菌诱导剂 XTH 和水杨酸调控马铃薯防御相关基因的表达

IF 2.3 3区 农林科学 Q1 AGRONOMY
Tiago Sartor, Natasha Ruschel Soares, Vítor da Silveira Falavigna, Luis Fernando Revers, Eliane R. Santarém, Leandro V. Astarita
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引用次数: 0

摘要

马铃薯(Solanum tuberosum)是一种主要的粮食作物,易受多种病原体和害虫的侵袭。利用生物诱导剂为作物管理提供了一种前景广阔的综合方法,有可能减少损失和化学处理。其中一种诱导剂是轴突黄单胞菌(XTH)的非活性悬浮液,以前曾被证明能增强马铃薯对病原体(如引起黑腿病的细菌)的抗性。然而,这种抗性的内在机制仍不清楚。因此,本研究调查了 XTH 对马铃薯植株防御代谢的影响,并将其与外源水杨酸(SA)(一种著名的防御信号分子)引起的反应进行了比较。我们分析了防御反应途径标记基因的表达,包括 JA/ET 响应基因(StPin2、StERF1 和 StJAZ1/TIFY10A-like)和 SA 响应基因(StPR-1b、StPR-2 和 StChtA)。用 SA 和 XTH 处理马铃薯植株,分析处理叶片和系统叶片。在最初的 24 小时内,除 StChtA 外,XTH 会上调局部和系统的所有分析基因。XTH 介导的 StPAL 和 Pin2 基因上调表明,这种诱导剂可能通过茉莉酸途径引发反应。外源施用 SA 可诱导马铃薯植株中 StPR、StChtA、StJAZ1/TIFY10A-like 和 StERF 的系统表达。我们的研究结果表明,XTH 通过同时激活水杨酸和茉莉酸信号途径来调节马铃薯植株中防御相关基因的表达。这种双重激活表明,XTH 可以成为马铃薯种植过程中作物管理的宝贵资源,有可能减少对化学农药的需求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bacterial Elicitor XTH and Salicylic Acid Regulate the Expression of Defence-Related Genes in Potato

Bacterial Elicitor XTH and Salicylic Acid Regulate the Expression of Defence-Related Genes in Potato

Potato (Solanum tuberosum) is a major staple food crop susceptible to numerous pathogens and pests. Utilising biotic elicitors offers a promising integrative approach for crop management, potentially reducing losses and chemical treatments. One such elicitor, an inactive suspension of Xanthomonas axonopodis (XTH), has previously been shown to enhance potato resistance against pathogens, like the bacterium causing blackleg disease. However, the underlying mechanism of this resistance remains unclear. Thus, this study investigated the effect of XTH on the defence metabolism in potato plants and compared it to the response elicited by exogenous salicylic acid (SA), a well-known defence-signalling molecule. We analysed the expression of marker genes for defence response pathways, including JA/ET-responsive genes (StPin2, StERF1, and StJAZ1/TIFY10A-like) and SA-responsive genes (StPR-1b, StPR-2, and StChtA). Potato plants were treated with either SA and XTH, and both treated and systemic leaves were analysed. XTH upregulated all analysed genes locally and systemically within the first 24 h, except for StChtA. The XTH-mediated upregulation of StPAL and Pin2 genes suggests this elicitor might trigger responses via the jasmonic acid pathway. Exogenous application of SA induced the systemic expression of StPR, StChtA, StJAZ1/TIFY10A-like, and StERF in potato plants. Our results indicate that XTH modulates the expression of defence-related genes in potato plants by simultaneously activating both the salicylic acid and jasmonic acid signalling pathways. This dual activation suggests that XTH could be a valuable resource for crop management in potato cultivation, potentially reducing the need for chemical pesticides.

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来源期刊
Potato Research
Potato Research AGRONOMY-
CiteScore
5.50
自引率
6.90%
发文量
66
审稿时长
>12 weeks
期刊介绍: Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as: Molecular sciences; Breeding; Physiology; Pathology; Nematology; Virology; Agronomy; Engineering and Utilization.
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