Qian Deng, Zeyu Dong, Zequan Chen, Zhuolin Shi, Ting Zhao, Xueying Guan, Yan Hu, Lei Fang
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引用次数: 0
摘要
果胶是植物细胞壁的主要成分。果胶酶(PEL,EC 4.2.2.2)利用反β-消除化学作用裂解果胶同半乳糖醛酸区域中的α-1,4糖苷键。然而,有关锦葵科植物中 PELs 的全面和进化分析的资料十分有限。在这项研究中,我们从 10 个锦葵科物种中鉴定了 597 个 PEL 基因。系统进化和主题分析表明,这些 PELs 可分为六个亚家族:支系 I、II、III、IV、Va 和 Vb。最大的两个亚家族,即支系 I 和支系 II 分别包含 237 和 222 个 PEL 成员。Va 和 Vb 支系的成员只包含 4 或 5 个图案,远远少于其他亚家族。基因复制分析表明,节段复制在 PEL 基因家族的物种扩展中起了关键作用。来自支系 I、IV、Va 和 Vb 的 PEL 在纤维伸长阶段均有表达,但支系 II 和 III 的几乎所有 PEL 基因在所调查的纤维发育阶段均无表达。我们进一步对 2 001 个登录基因和 229 个登录基因进行了单基因单倍型关联分析。有趣的是,14 个 PEL 与纤维长度和强度性状显著相关,而在纤维质量较好的......中,只有 8 个基因与纤维质量性状显著相关。我们的研究结果为进一步研究 PEL 基因家族成员的进化和功能及其在棉花纤维品质改良中的潜在用途提供了重要信息。
Genome-wide identification of the pectate lyase (PEL) gene family members in Malvaceae, and their contribution to cotton fiber quality
Pectin is a major constituent of the plant cell wall. Pectate lyase (PEL, EC 4.2.2.2) uses anti-β-elimination chemistry to cleave the α-1,4 glycosidic linkage in the homogalacturonan region of pectin. However, limited information is available on the comprehensive and evolutionary analysis of PELs in the Malvaceae. In this study, we identified 597 PEL genes from 10 Malvaceae species. Phylogenetic and motif analyses revealed that these PELs are classified into six subfamilies: Clades I, II, III, IV, Va, and Vb. The two largest subfamilies, Clades I and II, contained 237 and 222 PEL members, respectively. The members of Clades Va and Vb only contained four or five motifs, far fewer than the other subfamilies. Gene duplication analysis showed that segmental duplication played a crucial role in the expansion of the PEL gene family in species. The PELs from Clades I, IV, Va, and Vb were expressed during the fiber elongation stage, but nearly all PEL genes from Clades II and III showed no expression in any of the investigated fiber developmental stages. We further performed single-gene haplotype association analysis in 2,001 accessions and 229 accessions. Interestingly, 14 PELs were significantly associated with fiber length and strength traits in with superior fiber quality, while only eight genes were found to be significantly associated with fiber quality traits in . Our findings provide important information for further evolutionary and functional research on the PEL gene family members and their potential use for fiber quality improvement in cotton.
期刊介绍:
Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.