Juliana M. E. Lima, Iasminy S. Santos, Joyce P. Alvarenga, Robert M. Gutiérrez, Lillian M. Azevedo, Thaís A. Sales, Marlon E. López, Kellen K. P. de Oliveira, André A. Lima, Teodorico C. Ramalho, Antonio Chalfun-Junior
{"title":"1-MCP 触发咖啡开花及其对不同乙烯受体的结合亲和力和基因表达的影响","authors":"Juliana M. E. Lima, Iasminy S. Santos, Joyce P. Alvarenga, Robert M. Gutiérrez, Lillian M. Azevedo, Thaís A. Sales, Marlon E. López, Kellen K. P. de Oliveira, André A. Lima, Teodorico C. Ramalho, Antonio Chalfun-Junior","doi":"10.1007/s00344-024-11481-x","DOIUrl":null,"url":null,"abstract":"<p>Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction <i>in silico</i> between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (<i>CaERS1-like</i>, <i>ETR2-like</i>, <i>ETR4-like</i> and <i>EIN4-like</i>), increasing it in leaves (<i>ETR1-like</i>, <i>ETR2-like</i>, <i>ETR4-like</i> and <i>CaCTR1-like</i>); and increasing the expression of <i>CaACO3-like</i> (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.</p>","PeriodicalId":16842,"journal":{"name":"Journal of Plant Growth Regulation","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors\",\"authors\":\"Juliana M. E. Lima, Iasminy S. Santos, Joyce P. Alvarenga, Robert M. Gutiérrez, Lillian M. Azevedo, Thaís A. Sales, Marlon E. López, Kellen K. P. de Oliveira, André A. Lima, Teodorico C. Ramalho, Antonio Chalfun-Junior\",\"doi\":\"10.1007/s00344-024-11481-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction <i>in silico</i> between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (<i>CaERS1-like</i>, <i>ETR2-like</i>, <i>ETR4-like</i> and <i>EIN4-like</i>), increasing it in leaves (<i>ETR1-like</i>, <i>ETR2-like</i>, <i>ETR4-like</i> and <i>CaCTR1-like</i>); and increasing the expression of <i>CaACO3-like</i> (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.</p>\",\"PeriodicalId\":16842,\"journal\":{\"name\":\"Journal of Plant Growth Regulation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Growth Regulation\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00344-024-11481-x\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Growth Regulation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00344-024-11481-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors
Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction in silico between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (CaERS1-like, ETR2-like, ETR4-like and EIN4-like), increasing it in leaves (ETR1-like, ETR2-like, ETR4-like and CaCTR1-like); and increasing the expression of CaACO3-like (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.