1-MCP 触发咖啡开花及其对不同乙烯受体的结合亲和力和基因表达的影响

IF 3.9 3区 生物学 Q1 PLANT SCIENCES
Juliana M. E. Lima, Iasminy S. Santos, Joyce P. Alvarenga, Robert M. Gutiérrez, Lillian M. Azevedo, Thaís A. Sales, Marlon E. López, Kellen K. P. de Oliveira, André A. Lima, Teodorico C. Ramalho, Antonio Chalfun-Junior
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引用次数: 0

摘要

乙烯是咖啡开花的关键调节因子,而 1-甲基环丙烯(1-MCP)是一种乙烯作用抑制剂,具有管理咖啡植物开花的潜力。本研究旨在确定 1-MCP 对乙烯受体基因表达的影响,并比较 1-MCP 与不同乙烯受体之间的相互作用,以了解该分子对咖啡开花的促进作用。研究涉及表型特征描述、乙烯定量、RNA 表达谱分析、系统进化分析、同源建模和分子对接。研究结果表明,1-MCP 可在 12 天内加速花芽发育至开花期,而用水处理的花芽则在降雨后才开始发育。此外,施用 1-MCP 还显著提高了叶片和花蕾中的乙烯水平。基因表达分析表明,1-氯丙二醇对叶片和花蕾中乙烯相关基因的表达有不同的调节作用。花蕾中信号基因(CaERS1-like、ETR2-like、ETR4-like 和 EIN4-like)的表达量减少,叶片中信号基因(ETR1-like、ETR2-like、ETR4-like 和 CaCTR1-like)的表达量增加;花蕾中 CaACO3-like(生物合成相关基因)的表达量增加。分子对接研究表明,乙烯可能对与 1-MCP 作用能量较低的受体具有较高的亲和力。总之,1-MCP 可能是通过调节乙烯相关过程来促进咖啡树开花的一种有前途的工具,因为 1-MCP 与乙烯受体之间的相互作用可能会通过对叶片和花蕾中基因表达的不同调节来影响乙烯敏感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

1-MCP Triggering Coffee Anthesis and Its Effects on Binding Affinity and Gene Expression of Different Ethylene Receptors

Ethylene is a pivotal regulator of coffee anthesis, and 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, offers a potential to manage flowering in coffee plants. This study aimed to identify the effect of 1-MCP on gene expression of ethylene receptors, and compare the interaction in silico between 1-MCP and different ethylene receptors to understand what could be associated with coffee anthesis promotion by this molecule. The study involved phenotypic characterization, ethylene quantification, RNA expression profiling, phylogenetic analysis, homology modeling, and molecular docking. Results revealed that 1-MCP accelerated floral bud development to anthesis within 12 days, contrasting with water treated floral buds which exhibited progress just after a rainfall event. Moreover, 1-MCP application notably increased ethylene levels in both leaves and floral buds. Gene expression analysis indicated that 1-MCP modulates ethylene related gene expression differently in leaves and floral buds. Decreasing the expression of signaling genes in floral buds (CaERS1-like, ETR2-like, ETR4-like and EIN4-like), increasing it in leaves (ETR1-like, ETR2-like, ETR4-like and CaCTR1-like); and increasing the expression of CaACO3-like (biosynthesis related gene) in floral buds. Molecular docking studies suggested that ethylene may exhibit a higher affinity for receptors with lower interaction energy with 1-MCP. In conclusion, 1-MCP could be a promising tool to promote anthesis in coffee trees by modulating ethylene related processes, as the interaction between 1-MCP and ethylene receptors may influence ethylene sensitivity through different modulation of gene expression in leaves and floral buds.

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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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