大蜡蛾的显微注射方案

James C Pearce, Amy Housden, Nicola J Senior, Olivia L Champion, Joann L Prior, Richard W Titball, James G Wakefield
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引用次数: 0

摘要

非脊椎动物 Galleria mellonella 3Rs 模型的一个局限性是缺乏遗传工具包。遗传可操作性的一个共同要求是向单细胞胚胎引入外源物质的方法,其中最常见的是显微注射。这篇短文介绍了一种饲养黄蜂姬鼠的详细方法,该方法可收集大量分期胚胎,并对胚胎进行去绒毛处理和显微注射,且死亡率有限。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A microinjection protocol for the greater waxworm moth, Galleria mellonella
A limitation to the non-vertebrate 3Rs model Galleria mellonella has been the lack of genetic toolkit. A common requirement for genetic tractability is a method to introduce exogenous material to the unicellular embryo, the most common of which is microinjection. This short article describes a detailed method for rearing Galleria mellonella to collect large amounts of staged embryos and to dechorionate and microinject embryos with limited mortality.
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