Tuo Li, Qin Wang, Yang Liu, Jiaguo Wang, Han Zhu, Linhua Cao, Dongyang Liu, Qirong Shen
{"title":"ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742 的 ADP-ribosylation factor GTPase-activating proteins 的不同作用","authors":"Tuo Li, Qin Wang, Yang Liu, Jiaguo Wang, Han Zhu, Linhua Cao, Dongyang Liu, Qirong Shen","doi":"10.1186/s13068-024-02570-w","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activating proteins (Arf-GAPs), pivotal in fungal morphogenesis, lack comprehensive studies on their regulatory mechanisms in lignocellulose utilization.</p><h3>Results</h3><p>Here, the orthologs (<i>Tg</i>Glo3 and <i>Tg</i>Gcs1) of Arf-GAPs in <i>S. cerevisiae</i> were characterized in <i>Trichoderma guizhouense</i> NJAU4742. The results indicated that overexpression of <i>Tggcs1</i> (OE-<i>Tggcs1</i>) enhanced the lignocellulose utilization, whereas increased expression of <i>Tgglo3</i> (OE-<i>Tgglo3</i>) elicited antithetical responses. On the fourth day of fermentation with rice straw as the sole carbon source, the activities of endoglucanase, cellobiohydrolase, xylanase, and filter paper of the wild-type strain (WT) reached 8.20 U mL<sup>−1</sup>, 4.42 U mL<sup>−1</sup>, 14.10 U mL<sup>−1</sup>, and 3.56 U mL<sup>−1</sup>, respectively. Compared to WT, the four enzymes activities of OE-<i>Tggcs1</i> increased by 7.93%, 6.11%, 9.08%, and 12.92%, respectively, while those decreased to varying degrees of OE-<i>Tgglo3</i>. During the nutritional growth, OE-<i>Tgglo3</i> resulted in the hyphal morphology characterized by sparsity and constriction, while OE-<i>Tggcs1</i> led to a notable increase in vacuole volume. In addition, OE-<i>Tggcs1</i> exhibited higher transport efficiencies for glucose and cellobiose thereby sustaining robust cellular metabolic rates. Further investigations revealed that <i>Tgglo3</i> and <i>Tggcs1</i> differentially regulated the transcription level of a dynamin-like GTPase gene (<i>Tggtp</i>), eliciting distinct redox states and apoptotic reaction, thus orchestrating the cellular response to lignocellulose utilization.</p><h3>Conclusions</h3><p>Overall, these findings underscored the significance of <i>Tg</i>Arf-GAPs as pivotal regulators in lignocellulose utilization and provided initial insights into their differential modulation of downstream targets.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"17 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02570-w","citationCount":"0","resultStr":"{\"title\":\"Divergent roles of ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742\",\"authors\":\"Tuo Li, Qin Wang, Yang Liu, Jiaguo Wang, Han Zhu, Linhua Cao, Dongyang Liu, Qirong Shen\",\"doi\":\"10.1186/s13068-024-02570-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activating proteins (Arf-GAPs), pivotal in fungal morphogenesis, lack comprehensive studies on their regulatory mechanisms in lignocellulose utilization.</p><h3>Results</h3><p>Here, the orthologs (<i>Tg</i>Glo3 and <i>Tg</i>Gcs1) of Arf-GAPs in <i>S. cerevisiae</i> were characterized in <i>Trichoderma guizhouense</i> NJAU4742. The results indicated that overexpression of <i>Tggcs1</i> (OE-<i>Tggcs1</i>) enhanced the lignocellulose utilization, whereas increased expression of <i>Tgglo3</i> (OE-<i>Tgglo3</i>) elicited antithetical responses. On the fourth day of fermentation with rice straw as the sole carbon source, the activities of endoglucanase, cellobiohydrolase, xylanase, and filter paper of the wild-type strain (WT) reached 8.20 U mL<sup>−1</sup>, 4.42 U mL<sup>−1</sup>, 14.10 U mL<sup>−1</sup>, and 3.56 U mL<sup>−1</sup>, respectively. Compared to WT, the four enzymes activities of OE-<i>Tggcs1</i> increased by 7.93%, 6.11%, 9.08%, and 12.92%, respectively, while those decreased to varying degrees of OE-<i>Tgglo3</i>. During the nutritional growth, OE-<i>Tgglo3</i> resulted in the hyphal morphology characterized by sparsity and constriction, while OE-<i>Tggcs1</i> led to a notable increase in vacuole volume. In addition, OE-<i>Tggcs1</i> exhibited higher transport efficiencies for glucose and cellobiose thereby sustaining robust cellular metabolic rates. Further investigations revealed that <i>Tgglo3</i> and <i>Tggcs1</i> differentially regulated the transcription level of a dynamin-like GTPase gene (<i>Tggtp</i>), eliciting distinct redox states and apoptotic reaction, thus orchestrating the cellular response to lignocellulose utilization.</p><h3>Conclusions</h3><p>Overall, these findings underscored the significance of <i>Tg</i>Arf-GAPs as pivotal regulators in lignocellulose utilization and provided initial insights into their differential modulation of downstream targets.</p></div>\",\"PeriodicalId\":494,\"journal\":{\"name\":\"Biotechnology for Biofuels\",\"volume\":\"17 1\",\"pages\":\"\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2024-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-024-02570-w\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology for Biofuels\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1186/s13068-024-02570-w\",\"RegionNum\":1,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology for Biofuels","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1186/s13068-024-02570-w","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Divergent roles of ADP-ribosylation factor GTPase-activating proteins in lignocellulose utilization of Trichoderma guizhouense NJAU4742
Background
The ability of lignocellulose degradation for filamentous fungi is always attributed to their efficient CAZymes system with broader applications in bioenergy development. ADP-ribosylation factor GTPase-activating proteins (Arf-GAPs), pivotal in fungal morphogenesis, lack comprehensive studies on their regulatory mechanisms in lignocellulose utilization.
Results
Here, the orthologs (TgGlo3 and TgGcs1) of Arf-GAPs in S. cerevisiae were characterized in Trichoderma guizhouense NJAU4742. The results indicated that overexpression of Tggcs1 (OE-Tggcs1) enhanced the lignocellulose utilization, whereas increased expression of Tgglo3 (OE-Tgglo3) elicited antithetical responses. On the fourth day of fermentation with rice straw as the sole carbon source, the activities of endoglucanase, cellobiohydrolase, xylanase, and filter paper of the wild-type strain (WT) reached 8.20 U mL−1, 4.42 U mL−1, 14.10 U mL−1, and 3.56 U mL−1, respectively. Compared to WT, the four enzymes activities of OE-Tggcs1 increased by 7.93%, 6.11%, 9.08%, and 12.92%, respectively, while those decreased to varying degrees of OE-Tgglo3. During the nutritional growth, OE-Tgglo3 resulted in the hyphal morphology characterized by sparsity and constriction, while OE-Tggcs1 led to a notable increase in vacuole volume. In addition, OE-Tggcs1 exhibited higher transport efficiencies for glucose and cellobiose thereby sustaining robust cellular metabolic rates. Further investigations revealed that Tgglo3 and Tggcs1 differentially regulated the transcription level of a dynamin-like GTPase gene (Tggtp), eliciting distinct redox states and apoptotic reaction, thus orchestrating the cellular response to lignocellulose utilization.
Conclusions
Overall, these findings underscored the significance of TgArf-GAPs as pivotal regulators in lignocellulose utilization and provided initial insights into their differential modulation of downstream targets.
期刊介绍：
Biotechnology for Biofuels is an open access peer-reviewed journal featuring high-quality studies describing technological and operational advances in the production of biofuels, chemicals and other bioproducts. The journal emphasizes understanding and advancing the application of biotechnology and synergistic operations to improve plants and biological conversion systems for the biological production of these products from biomass, intermediates derived from biomass, or CO2, as well as upstream or downstream operations that are integral to biological conversion of biomass.
Biotechnology for Biofuels focuses on the following areas:
• Development of terrestrial plant feedstocks
• Development of algal feedstocks
• Biomass pretreatment, fractionation and extraction for biological conversion
• Enzyme engineering, production and analysis
• Bacterial genetics, physiology and metabolic engineering
• Fungal/yeast genetics, physiology and metabolic engineering
• Fermentation, biocatalytic conversion and reaction dynamics
• Biological production of chemicals and bioproducts from biomass
• Anaerobic digestion, biohydrogen and bioelectricity
• Bioprocess integration, techno-economic analysis, modelling and policy
• Life cycle assessment and environmental impact analysis
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